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Tetracycline efficient degradation bacterium and application thereof

A technology of tetracycline and bacteria, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve problems affecting the popularization and application of microbial degradation methods, and achieve low application cost, good degradation effect, and high biological efficiency safety effect

Active Publication Date: 2018-03-30
YANCHENG TEACHERS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it has affected the popularization and application of microbial degradation method.

Method used

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  • Tetracycline efficient degradation bacterium and application thereof
  • Tetracycline efficient degradation bacterium and application thereof
  • Tetracycline efficient degradation bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Isolation, purification and preservation of strains

[0020] Take by weighing 5g to 50ml of organic manure fermented with chicken manure etc. as the raw material liquid culture medium containing tetracycline with different concentration gradients, and use tetracycline as the only carbon source (the medium formula is: every 1L of culture fluid contains 5g (NH4) 2 SO 4 , 1gKH 2 PO 4 , 0.5g MgSO 4 7H2O, 0.1g CaCl 2 , 0.1g NaCl), cultivated at 28°C and 150rpm for 7 days, and obtained a strain that can grow under the condition of using high concentration tetracycline as the sole carbon source through continuous enrichment culture according to the same culture conditions, and then absorbed 0.2% of The bacterial suspension is applied to a solid medium containing a higher concentration of tetracycline and used as a carbon source. After 2 to 3 days of culture, a typical single colony is picked for isolation and purification, and the final isolated pure culture strain is trea...

Embodiment 2

[0022] Effects of Different Carbon Sources, Nitrogen Sources, pH, Temperature and Inoculum Size on the Growth and Tetracycline Degradation of TR5

[0023] In the experimental group, glucose, starch, lactose and other sugars were used as the carbon source required for the growth of the strain, and yeast extract powder, peptone, casein, etc. were used as the nitrogen source required for the growth of the strain. ℃, the test was carried out under laboratory culture conditions with an inoculum size of 2% to 10%.

[0024] figure 1 The phylogenetic tree diagram of TR5.

[0025] figure 2 is the influence diagram of carbon source on the growth status and degradation efficiency of TR5, represented by figure 2 It can be seen that because sodium acetate is alkaline, and tetracycline is unstable and easy to decompose under alkaline conditions, the growth status of the TR5 strain is not good under this laboratory condition. Based on this figure, it is finally decided to use glucose as...

Embodiment 3

[0033] Degradability test

[0034] 1. Application of tetracycline-efficient degrading bacteria (TR5) in water purification of aquaculture industry

[0035] Get the water of a certain artificially cultured fish pond, after the water body is carried out preliminary purification treatment, measure the content of tetracycline in the water body with HPLC, be 200ppm, the moisture of fish pond is packed into culture bottle (every bottle 20ml), and in every bottle Add 20 pellets immobilized and embedded TR5 strain into the culture for culture, and set up a blank control group. After 5 days of culture, the content of tetracycline in the culture bottle was measured again, and it was found that compared with the control group, the experimental group had more The content has been significantly reduced, and the degradation efficiency of the experimental group is 86%.

[0036] 2. Application of tetracycline-efficient degrading bacteria in wastewater treatment of tetracycline antibiotic pha...

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Abstract

The invention discloses a tetracycline efficient degradation bacterium and application thereof. One strain of the tetracycline efficient degradation bacterium is obtained through screening from organic fertilizers fermented by using chicken manure and the like as raw materials; through physiological-biochemical characteristics and 16SrDNA sequence analysis, the strain is identified to be Klebsiella Trevisan, and is named as TR5. The tetracycline degradation efficiency of the used strain is forecasted to reach 90 percent or higher through response surface experiment design at a laboratory level. The value is improved by about 20 percent through being compared with the data reported in most documents. A microbial immobilization technology is also used for improving the anti-impact load capability of the tetracycline efficient degradation bacterium in a water body and the organism quantity; a green and environment-pollution-free biological path is provided for the relevant aquaculture water body treatment and pharmaceutical factory tetracycline antibiotic sewage control.

Description

technical field [0001] The present invention relates to a bacterial strain of tetracycline-degrading bacteria Klebsiella genus, in particular to the application of a microbial immobilization embedding technology to the purification of aquaculture water and the treatment of tetracycline antibiotic wastewater in pharmaceutical factories. Background technique [0002] In recent years, the problem of environmental pollution caused by the extensive use of tetracycline antibiotics in medical treatment and livestock and poultry breeding has become increasingly serious, and has become one of the hot spots of research at home and abroad. Studies have reported that in addition to causing chemical pollution, antibiotics in the environment may also induce the production of resistant microorganisms and resistance genes in the environment, and aggravate the risk of antibiotic resistance transmission. Some resistant microorganisms may directly or indirectly participate in the food chain an...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/22C02F101/38C02F103/20C02F103/34
CPCC02F3/34C02F2101/38C02F2103/20C02F2103/343C12N1/205C12R2001/22
Inventor 康贻军夏丹印志峰沈敏赵庆新张言周
Owner YANCHENG TEACHERS UNIV
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