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A kind of human sperm acrosome enzyme kit

An acrosome enzyme and sperm technology, applied in the field of human sperm acrosome enzyme kits, can solve the problems of complex operation and high cost

Active Publication Date: 2021-07-06
高晓勤 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] To sum up, the problems existing in the prior art are: the current method for detecting sperm acrosome enzyme is complex in operation and high in cost.

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  • A kind of human sperm acrosome enzyme kit
  • A kind of human sperm acrosome enzyme kit
  • A kind of human sperm acrosome enzyme kit

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Embodiment Construction

[0028] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0029] Andrology is an emerging discipline that studies male reproductive activities, and is one of the important areas to be developed in contemporary medical science. Infertility is a worldwide problem. According to the WHO survey, 15% of couples of childbearing age have infertility problems. The production and transportation of male sperm is a complex physiological process. There are many factors that lead to infertility, such as environmental factors, endocrine dysfunction, Testicular spermatogenesis dysfunction, abnormal sperm structure and function, etc., with the deepening of research, the research on sperm enzymolog...

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Abstract

The invention belongs to the technical field of sperm acrosinase measurement, and discloses a human sperm acrosinase kit. The gelatin mixture is made into a sol-like matrix liquid, which is coated and fixed into a uniform base film, and the acroinase (Acroin) is displayed. Activity; spermatozoa are incubated on an ultra-thin gelatin base membrane, centrifuged and washed with phosphate buffer to remove the membrane inhibitor sperm acrosome depolymerization, release and dissolve-gelatin matrix membrane, forming a pore area around the front of the spermatozoon; by Light microscope observation, the light penetrates the hole area, and a bright halo is seen around the sperm head. Against the background of dark purple blue after staining, the enzyme reaction is observed, and the diameter of each bright ring is measured to estimate a single sperm The strength of acrosome enzyme activity. The method of the invention is simple and low in cost, accurately calculates the positive reaction rate of the acrosome enzyme, screens out more active sperm for the test-tube baby technique, and improves the success rate.

Description

technical field [0001] The invention belongs to the technical field of sperm acrosome enzyme measurement, in particular to a human sperm acrosome enzyme kit. Background technique [0002] Human sperm acrosome contains 24 enzymes known to humans, among which hyaluronidase (Acrosin) and acrosin (Acrosin) are most closely related to fertilization. Acrosin plays an important role in the combination of sperm and egg during fertilization. Determination of sperm acrosin (Acrosin) activity is one of the indicators to evaluate sperm function. In the previous research methods on sperm acrosome enzymes, most of them used biochemical methods to detect the enzyme activity, positive rate and enzyme reaction time of all sperm in the whole sample. For example, the determination of sperm acrosome arginine amidase activity requires expensive reagents such as polyethylene glycol octylphenyl ether (Triton solution), benzoyl arginyl p-nitroanilide (BAPNA solution), and can only react The acro...

Claims

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Application Information

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IPC IPC(8): C12Q1/37
CPCC12Q1/37
Inventor 高晓勤戴研平马晓萍杨喆杨建辉郑立宏杨丹杨燕平
Owner 高晓勤
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