Method for rapid gelation of silk protein solution under physiological conditions

A technology of silk protein and physiological conditions, applied in the field of rapid gelation of silk protein solution, can solve the problems of large cytotoxicity, difficult to degrade, accelerate the gelation rate of silk protein solution, etc., and achieve good biocompatibility and good biological properties. Compatibility effect

CN107892755AInactive Publication Date: 2018-04-10SUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2018-04-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for rapid gelation of a silk protein solution under physiological conditions. The method comprises the following steps: (1) weighing NapFF solid powder to put onto thebottom of a glass vial, and adding ultrapure water; (2) adding a NaOH solution, performing ultrasonic auxiliary dissolution, and using a precise temperature control micro-computer electro-heating plate for heating the bottom of the vial at 70 DEG C for 1-2 min to form a transparent solution; (3) slowly adding a HCl solution, and stirring well with a pipette until solution pH is 7.2-7.5; (4) addingthe silk protein solution; (5) adding the ultrapure water to a metered volume of 200 mu L; and (6) placing horizontally, and observing the gelation process by tilting and inverting the glass vial. The method can induce the rapid gelation of the low-concentration silk protein solution in a short period of time, and the gelatinized silk protein solution can be degraded hydrolysis in the body by useof proteolytic enzyme, does not produce toxic and side effects, has good biocompatibility, and can be used as a good biomaterial.
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Description

technical field

[0001] The invention relates to a method for rapid gelation of silk protein solution under physiological conditions. Background technique

[0002] Silk Fibroin, also known as silk fibroin, is a natural high-molecular fiber protein extracted from silk, and its content accounts for about 70% to 80% of silk. It contains 18 kinds of amino acids, including glycine (gly) and alanine ( ala) and serine (ser) accounted for more than 80% of the total content. Silk fibroin itself has good mechanical properties and physical and chemical properties, such as good flexibility and tensile strength, breathable and moisture permeability, slow release, etc., and different forms can be obtained after different treatments, such as nanofibers, nanoparticles, silk Fibroin films, sponge-like porous scaffold materials, silk fibroin hydrogels and various materials made by electrospinning, etc. Due to its slow biodegradation, good biocompatibility, and low immunogenicity, silk protei...

Examples

Embodiment 1

[0060] A method for rapid gelation of silk protein solution under physiological conditions, comprising the following steps:

[0061] (1) Weigh 0.4 mg of NapFF solid powder into the bottom of a glass vial, and add 170 μL of ultrapure water;

[0062] (2) Add 8 μL of 1 mol / L NaOH solution prepared with ultrapure water, dissolve with ultrasonic assistance, and heat the bottom of the vial at 70°C for 1 min using a microcomputer electric heating plate that can precisely control the temperature to form a transparent solution;

[0063] (3) Slowly add 1 mol / L HCl solution prepared with ultrapure water, and stir well with a pipette tip until the pH of the solution is 7.4;

[0064] (4) Add 1.7 μL of silk protein solution with a concentration of 12.03wt%;

[0065] (5) Add ultrapure water to make the volume to 200μL;

[0066] (6) Place it horizontally, and observe the gelation process by tilting and inverting the glass vial every 10 minutes.

[0067] After standing at room temperature f...

Embodiment 2

[0069] A method for rapid gelation of silk protein solution under physiological conditions, comprising the following steps:

[0070] (1) Weigh 0.8 mg of NapFF solid powder into the bottom of a glass vial, and add 170 μL of ultrapure water;

[0071] (2) Add 10 μL of 1 mol / L NaOH solution prepared with ultrapure water, dissolve with ultrasonic assistance, and heat the bottom of the vial at 70°C for 2 minutes using a microcomputer electric heating plate that can precisely control the temperature to form a transparent solution;

[0072] (3) Slowly add 1 mol / L HCl solution prepared with ultrapure water, and stir well with a pipette tip until the pH of the solution is 7.4;

[0073] (4) Add 1.7 μL of silk protein solution with a concentration of 12.03wt%;

[0074] (5) Add ultrapure water to make the volume to 200μL;

[0075] (6) Place it horizontally, and observe the gelation process by tilting and inverting the glass vial every 5 minutes.

[0076] It is found that after 30 minute...

Embodiment 3

[0078] A method for rapid gelation of silk protein solution under physiological conditions, comprising the following steps:

[0079] (1) Weigh 0.8 mg of NapFF solid powder into the bottom of a glass vial, and add 150 μL of ultrapure water;

[0080] (2) Add 12 μL of 1 mol / L NaOH solution prepared with ultrapure water, dissolve with the aid of ultrasound, and heat the bottom of the vial at 70°C for 2 minutes using a microcomputer electric heating plate that can precisely control the temperature to form a transparent solution;

[0081] (3) Slowly add 1 mol / L HCl solution prepared with ultrapure water, and stir well with a pipette tip until the pH of the solution is 7.4;

[0082] (4) Add 8.3 μL of silk protein solution with a concentration of 12.03wt%;

[0083] (5) Add ultrapure water to make the volume to 200μL;

[0084] (6) Place it horizontally, and observe the gelation process by tilting and inverting the glass vial every 5 minutes.

[0085] It was found that within 10 minu...