Method for rapid gelation of silk protein solution under physiological conditions

Abstract

The invention provides a method for rapid gelation of a silk protein solution under physiological conditions. The method comprises the following steps: (1) weighing NapFF solid powder to put onto thebottom of a glass vial, and adding ultrapure water; (2) adding a NaOH solution, performing ultrasonic auxiliary dissolution, and using a precise temperature control micro-computer electro-heating plate for heating the bottom of the vial at 70 DEG C for 1-2 min to form a transparent solution; (3) slowly adding a HCl solution, and stirring well with a pipette until solution pH is 7.2-7.5; (4) addingthe silk protein solution; (5) adding the ultrapure water to a metered volume of 200 mu L; and (6) placing horizontally, and observing the gelation process by tilting and inverting the glass vial. The method can induce the rapid gelation of the low-concentration silk protein solution in a short period of time, and the gelatinized silk protein solution can be degraded hydrolysis in the body by useof proteolytic enzyme, does not produce toxic and side effects, has good biocompatibility, and can be used as a good biomaterial.

Description

technical field [0001] The invention relates to a method for rapid gelation of silk protein solution under physiological conditions. Background technique [0002] Silk Fibroin, also known as silk fibroin, is a natural high-molecular fiber protein extracted from silk, and its content accounts for about 70% to 80% of silk. It contains 18 kinds of amino acids, including glycine (gly) and alanine ( ala) and serine (ser) accounted for more than 80% of the total content. Silk fibroin itself has good mechanical properties and physical and chemical properties, such as good flexibility and tensile strength, breathable and moisture permeability, slow release, etc., and different forms can be obtained after different treatments, such as nanofibers, nanoparticles, silk Fibroin films, sponge-like porous scaffold materials, silk fibroin hydrogels and various materials made by electrospinning, etc. Due to its slow biodegradation, good biocompatibility, and low immunogenicity, silk protei...

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Problems solved by technology

However, these additional conditions are greatly restricted in the practical application of silk protein as a biomaterial.

Scientific researchers also adjust organic small molecular substances or high molecular polymers (for example: ethanol, surfactant sodium dodecylsulfonate or hydrophilic polymer polyethylene glycol, etc.) to the silk protein solution. It interacts with the silk protein chains to accelerate the gelation rate of the silk protein solution, but this series of gelation processes may not be compatible with some clinical use environments, such as the potential cell and tissue toxicity of organic molecules ; The bioinertness of high molecular polymers, difficult to degrade in vivo and other characteristics, although these measures can shorten the gelation time of silk protein to a certain extent, but the biocompatibility of the obtained silk protein gel is poor, and the larger Cytotoxicity, these problems have greatly limited its application in biomedical materials

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