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Injectable moulded silk fibroin hydrogel and preparation method thereof

A silk fibroin and hydrogel technology, applied in biochemical equipment and methods, non-active components of polymer compounds, immobilized on/in organic carriers, etc., can solve the toxicity and reduce the biocompatibility of silk fibroin It is easy to inject, avoid trauma, and has good biocompatibility.

Active Publication Date: 2012-12-05
JIANGSU HUAJIA SILK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As we all know, epoxy compounds have certain toxicity. Although the unreacted epoxy crosslinking agent is removed by water washing, there is still residual epoxy crosslinking agent in the gel, which brings certain toxicity and reduces the viscosity of silk fibroin. Biocompatibility ([J] Journal of Biomedical Engineering, 2007, 24 (6): 1309~1313)
Chinese invention patent (CN101502670) discloses a preparation method of silk fibroin hydrogel, which can form hydrogel in a short time by using ultrasonic vibration method, but it is difficult to achieve injectable and in vivo molding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation steps of the silk fibroin hydrogel provided by the present embodiment are as follows:

[0019] 1. Put 40 grams of white plant silk into 4 liters of sodium carbonate solution with a mass concentration of 0.05%, and treat it at 98-100°C for 0.5 hours, then wash it, and put it into 4 liters of sodium carbonate solution with a concentration of 0.05% again. Treat at 98-100°C for 0.5 hours, repeat 3 times to degumming silk, wash and dry fully to obtain pure silk fiber. Dissolve 300 ml of pure silk fiber with 9.3 mol / L lithium bromide solution at 65±2°C to form a mixed silk fibroin solution;

[0020] 2. Put the resulting mixed silk fibroin solution into a dialysis bag, and dialyze with deionized water for 3 days to remove impurities such as lithium bromide to obtain a pure silk fibroin solution;

[0021] 3. Adjust the concentration of silk fibroin solution to 30g / l, which is component A, and store it at a temperature of 4°C;

[0022] 4. Prepare a so...

Embodiment 2

[0025] 1. Put 40 grams of raw silk into 5 liters of 0.2% sodium carbonate solution, treat it at 98-100° C. for 2 hours to degumming the silk, wash and dry it fully to obtain pure silk fiber. Put pure silk fiber in 300 ml of lithium bromide solution with a concentration of 9.3 mol / L, stir and dissolve at a temperature of 65±2°C to form a mixed silk fibroin solution;

[0026] 2. Put the resulting mixed silk fibroin solution into a dialysis bag, and dialyze with deionized water for 3 days to remove impurities such as lithium bromide to obtain a pure silk fibroin solution;

[0027] 3. Adjust the concentration of silk fibroin solution to 20 g / l as component A, and store it at 6°C;

[0028] 4. Prepare sodium lauryl sulfate solution with a concentration of 20 g / l as component B and store at 6°C;

[0029] 5. Mix the above-mentioned component A and component B at a volume ratio of A:B=10:1, inject the mixed solution into the body with a syringe, and form the desired shape of...

Embodiment 3

[0032] 1. Put 100 grams of waste silk into 4 liters of neutral soap solution with a concentration of 0.05%, degumming at 98-100°C for 0.5 hours, repeat the treatment 3 times, and obtain pure silk fiber after fully washing. Heat and dissolve the dried pure silk fiber with 100 ml of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 at 75±5°C to obtain a silk fibroin mixed solution;

[0033] 2. Put the obtained silk fibroin mixed solution into a dialysis bag, and dialyze with deionized water for 3 days to remove impurities such as calcium chloride and ethanol to obtain a pure silk fibroin solution;

[0034] 3. Adjust the concentration of silk fibroin solution to 40g / l as component A; store it at a temperature of 8°C;

[0035] 4. Prepare sodium octadecyl sulfate with a concentration of 10 g / l, and Poloxamer188 solution with a concentration of 1 g / l as component B; store at a temperature of 8°C;

[0036] 5. Mix the above-mentioned components A and ...

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Abstract

The invention relates to a hydrogel material and a preparation method thereof, and particularly relates to an injectable moulded silk fibroin hydrogel and a preparation method thereof. The method comprises the following steps: storing a silk fibroin water solution having a concentration of 10 to 300 g / l and a surfactant solution having a concentration of 0.01 to 100 g / l at a temperature of 2 to 8DEG C, evenly mixing the two solutions according to a volume ratio of 10:1, and standing the mixed solution at a temperature of 30 to 40 DEG C for 5 to 30 minutes; therefore, an injectable moulded silk fibroin hydrogel is obtained. The injectable moulded silk fibroin hydrogel has good biocompatibility and is applicable to biomedical fields such as artificial skin, artificial cartilage, cell culture support, bioreactors, enzyme immobilized materials and pharmaceutical accessories. Particularly, it is convenient for the silk fibroin hydrogel to form in vivo in situ, thereby avoiding wounds by surgery; therefore, the injectable moulded silk fibroin hydrogel is particularly applicable to the restoration of tissues of a complex shape.

Description

technical field [0001] The invention relates to a hydrogel material and a preparation method thereof, in particular to an injection-molded silk fibroin hydrogel, which can be used as tissue repair materials, pharmaceutical auxiliary materials, etc., and applied in the field of biomedicine. Background technique [0002] Gel is a physical state between solid and liquid, and has unique properties, especially hydrogel, which is soft, can maintain a certain shape, and maintains a large amount of water in it. The physical cross-linking and chemical cross-linking will not dissolve in water, but can only swell and maintain a certain shape. Hydrogels have good biocompatibility and water permeability, and are currently widely used in artificial skin, tissue engineering materials, drug release, artificial muscles, biosensors, separation devices, and recovery of heavy metal ions. Injectable hydrogels can be formed in situ in the body, which can avoid highly traumatic surgical proced...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L89/00C08J3/075A61L27/22A61L27/60A61L31/04A61K47/42C12N5/00C12N11/02
Inventor 卢神州李明忠吴锡龙
Owner JIANGSU HUAJIA SILK
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