Method for direct and rapid propagation of bletilla striata by soil culture

A technology of seeds and seedlings, which is applied in the field of direct seeding and rapid propagation of white and soil culture, can solve the problems of increasing cost investment, and achieve the effects of low cost, developed root system and pseudobulb, and increased air permeability

Inactive Publication Date: 2018-04-13
ZUNYI MEDICAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

Tissue culture is currently the most commonly used artificial breeding method. However, the use of this tech

Method used

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  • Method for direct and rapid propagation of bletilla striata by soil culture

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preparation example Construction

[0020] In the present invention, the aseptic white and the seeds are derived from the sterile white and the capsule, and the sterile white and the capsule are cut to obtain the sterile white and the seed. In the present invention, the preparation method of aseptic bletilla capsules comprises the following steps: A) after wiping the surface of mature bacilli capsules with alcohol, wash with sterile water to obtain pre-sterilized bletilla capsules; B) soak in mercuric acid for pre-sterilization After the white and capsules were obtained, they were washed with sterile water to obtain sterile white and capsules.

[0021] In the present invention, the ripe white and capsule color is slightly yellow; the volume concentration of the alcohol is preferably 75%; in the present invention, it is preferred to use absorbent cotton dipped in alcohol to wipe the surface of the mature white and capsule. After wiping with alcohol, wash with aseptic water to remove alcohol, the number of times o...

Embodiment 1

[0032] Step 1. Preparation of Baihe sterile capsules: Select mature Baiji capsules that grow to light yellow, wipe their surface with absorbent cotton soaked in 75% alcohol, wash them twice with sterile water, and then soak them in 0.1% mercuric chloride 8min, washed 3 times with sterile water, and removed the water on the surface of the capsule with sterile dry absorbent paper for later use.

[0033]Step 2. Preliminary germination of white and seeds: take a 10ml plastic EP tube that has been sterilized by damp heat method (121°C, pressure 0.1-1.0MPa, sterilized for 20min), and dried, and add 10ml sterile distilled water to it , then cut off the top of the capsule in step 1 with sterile scissors, gently shake the white and seeds into the EP tube, cover the lid, and put it under 18h / 6h (light / dark) light at 24±1°C Cultivate for 2 days under the condition;

[0034] Step 3. Sowing: Mix the imported peat soil, local loess, shredded coconut, and vermiculite in a mass ratio of 5:3:...

Embodiment 2

[0039] Step 1. Preparation of Baiji sterile capsules: Select mature Baiji capsules that grow to light yellow, wipe their surface with 75% alcohol absorbent cotton, wash once with sterile water, and then soak them in 0.1% mercuric chloride 9min, wash 5 times with sterile water, and remove the water on the surface of the capsule with sterile dry absorbent paper for later use.

[0040] Step 2. Preliminary germination of white and seeds: take a 10ml plastic EP tube that has been sterilized by damp heat method (121°C, pressure 0.1-1.0MPa, sterilized for 20min), and dried, and add 10ml sterile distilled water to it , then cut off the top of the capsule in step 1 with sterile scissors, gently shake the white and seeds into the EP tube, cover the lid, and store it under the light conditions of 24°C, 18h / 6h (light / dark). culture 3d;

[0041] Step 3. Sowing: Mix the imported peat soil, local loess, coconut shreds, and vermiculite in a mass ratio of 4:3:2:0.5, and put 3L of mixed soil i...

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Abstract

The invention provides a method for direct and rapid propagation of bletilla striata through soil culture. The method comprises the following steps of 1, germinating sterile bletilla striata seeds insterile water to obtain germinating seeds; 2, spreading the germinating seeds into a matrix and placing the matrix in an environment of 23 to 25 DEG C for natural light culture for 4 to 6 weeks to obtain bletilla striata young seedlings; 3, culturing the bletilla striata young seedlings for 2 to 8 weeks to obtain bletilla striata seedlings and spraying a plant full-nutrient solution every 2 to 4 days in the process of obtaining the bletilla striata seedlings through culture. According to the method, the seed germination rate can be increased to 70-90%; the seedling rate can reach 60-70%; the obtained bletilla striata seedlings have more developed roots and pseudo-bulbs, and the quality of the bletilla striata seedlings is better.

Description

technical field [0001] The invention belongs to the technical field of baiji breeding, and in particular relates to a method for direct direct seeding and rapid propagation of baiji soil cultivation. Background technique [0002] Baiji (Bletilla striata (Thunb.) Reichb.f.) is a perennial herb belonging to Orchidaceae Baiji, also known as "Bletilla striata". It is a famous and precious Chinese herbal medicine in my country. Pharmacopoeia Commission, 2010), modern pharmacological research shows that it has anti-cancer, anti-oxidation, antibacterial and other effects. Baiji is also an important industrial feedstock, and with the increase of applications, the market demand is gradually expanding. [0003] However, due to the huge market demand, the wild Baihe has been almost excavated, and it has become a rare and precious Chinese medicinal material that is endangered. be protected. How to protect the germplasm resources of Baiji and how to obtain a large number of seedlings i...

Claims

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Application Information

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IPC IPC(8): A01G22/00A01G22/25A01G24/15A01G24/22A01G24/28A01C1/00A01C21/00
CPCA01C1/00A01C21/005
Inventor 李林蔡治垠徐德林葛华顺钱刚罗才林郑明辉上官燕妮谢欣
Owner ZUNYI MEDICAL UNIVERSITY
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