Preparation method of liquid compound enzymes for monogastric animals

A liquid compound enzyme, liquid technology, used in animal feed, additional food elements, applications, etc.

Inactive Publication Date: 2018-04-13
JINANBESTZYME BIO ENG CO LTD
View PDF13 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for preparing monogastric animal liquid compound enzyme, which solves the problems in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of liquid compound enzymes for monogastric animals
  • Preparation method of liquid compound enzymes for monogastric animals
  • Preparation method of liquid compound enzymes for monogastric animals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of monogastric animal liquid compound enzyme adopts the following steps:

[0022] (1) Liquid xylanase and liquid thermostable phytase are finely filtered respectively, and the specific operations are:

[0023] Use a 20% mass fraction of sodium hydroxide solution to adjust the pH of the liquid xylanase product to 6.00-7.00, incubate at 30°C-40°C for 2 hours, add 10# diatomaceous earth with 2% mass fraction of the liquid xylanase to filter, The fine filtrate is adjusted to pH 4.50-5.50 with glacial acetic acid;

[0024] Adjust the pH of the liquid heat-resistant phytase product to 6.50-7.00 with sodium hydroxide solution, keep it warm at 35°C-40°C for 2 hours, add 10# diatomaceous earth with 2% of the liquid heat-resistant phytase quality, filter it, and use glacial acetic acid Adjust the pH of the filtrate to 4.50-5.50;

[0025] After the above-mentioned fine filtration treatment, remove miscellaneous proteins and other associated enzymes in the pr...

Embodiment 2

[0035] A preparation method of monogastric animal liquid compound enzyme adopts the following steps:

[0036] (1) Liquid xylanase and liquid thermostable phytase are finely filtered respectively, and the specific operations are:

[0037] Use a 20% mass fraction of sodium hydroxide solution to adjust the pH of the liquid xylanase product to 6.00-7.00, incubate at 30°C-40°C for 2 hours, add 10# diatomaceous earth with 2% mass fraction of the liquid xylanase to filter, The fine filtrate is adjusted to pH 4.50-5.50 with glacial acetic acid;

[0038] Adjust the pH of the liquid heat-resistant phytase product to 6.50-7.00 with sodium hydroxide solution, keep it warm at 35°C-40°C for 2 hours, add 10# diatomaceous earth with 2% of the liquid heat-resistant phytase quality, filter it, and use glacial acetic acid Adjust the pH of the filtrate to 4.50-5.50;

[0039] After the above-mentioned fine filtration treatment, remove miscellaneous proteins and other associated enzymes in the pr...

Embodiment 3

[0049] A preparation method of monogastric animal liquid compound enzyme adopts the following steps:

[0050] (1) Liquid xylanase and liquid thermostable phytase are finely filtered respectively, and the specific operations are:

[0051] Use a 20% mass fraction of sodium hydroxide solution to adjust the pH of the liquid xylanase product to 6.00-7.00, incubate at 30°C-40°C for 2 hours, add 10# diatomaceous earth with 2% mass fraction of the liquid xylanase to filter, The fine filtrate is adjusted to pH 4.50-5.50 with glacial acetic acid;

[0052] Adjust the pH of the liquid heat-resistant phytase product to 6.50-7.00 with sodium hydroxide solution, keep it warm at 35°C-40°C for 2 hours, add 10# diatomaceous earth with 2% of the liquid heat-resistant phytase quality, filter it, and use glacial acetic acid Adjust the pH of the filtrate to 4.50-5.50;

[0053] After the above-mentioned fine filtration treatment, remove miscellaneous proteins and other associated enzymes in the pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of preparation of an enzyme preparation, in particular to a preparation method of liquid compound enzymes for monogastric animals. The preparation method of the liquid compound enzymes for the monogastric animals comprises the following steps of firstly, performing fine filtration treatment on liquid xylanase and liquid heat-resisting phytase, adding liquid cellulases, liquid beta-glucanase and liquid acidic mannase in proportion according to specific steps, performing stirring, then adding different types of stabilizing agents and preservatives, regulating different pH of a mixed solution, accelerating the experiment, and tracking and detecting the enzyme activity and appearance changes, so that the liquid compound enzymes being low in enzyme activity loss and stable in product quality for the monogastric animals are obtained.

Description

Technical field: [0001] The invention relates to the technical field of enzyme preparation, in particular to a method for preparing monogastric animal liquid compound enzyme. Background technique: [0002] In my country’s feed industry, in addition to corn-soybean meal-based rations, 10%-40% of barley or wheat, secondary flour, wheat bran, and traditional Unconventional feed materials such as bran and cottonseed meal can replace 50% to 60% of corn. However, non-starch polysaccharides and phytate phosphorus in grains are difficult to be digested and absorbed by monogastric animals. Non-starch polysaccharides can increase the viscosity of chyme in the digestive tract, resulting in reduced nutrient digestibility and feeding effects in the diet. Phytate phosphorus in feed is difficult to utilize and requires Additional mineral phosphorus is added, but the digestibility of phosphorus is very low, resulting in excretion of excess phosphorus in the feces of monogastric animals, whi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23K20/189
CPCA23K20/189
Inventor 赵素珍李文彬刘延杰宋迪
Owner JINANBESTZYME BIO ENG CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap