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Method of extracting and separating myricetin and dihydromyricetin from shuteria pampaniniana

A technology of dihydromyricetin and grass red, applied in the field of medicine, can solve the problems of poor handling of the extract, high material consumption, complicated process, etc., and achieve the effect of reducing the cost of raw materials

Inactive Publication Date: 2018-04-13
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the separation methods of myricetin and dihydromyricetin in S. chinensis reported in domestic literature mainly adopt water decoction and polyamide column separation. This method is complex in process, difficult to handle the extract, high in material consumption, and low in yield (Cao Yanhuai, Huang Yuanzheng, Ding Lisheng. Flavonoids in Radix Sargentina[J]. China Journal of Traditional Chinese Medicine, 2000, 25(5): 290-291)

Method used

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  • Method of extracting and separating myricetin and dihydromyricetin from shuteria pampaniniana
  • Method of extracting and separating myricetin and dihydromyricetin from shuteria pampaniniana
  • Method of extracting and separating myricetin and dihydromyricetin from shuteria pampaniniana

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Experimental program
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Embodiment 1

[0018] (1) Take 100 g of red rattan powder, add 80% ethanol to heat and reflux for extraction three times, concentrate the ethanol extract, add distilled water to disperse it, extract twice with ethyl acetate, concentrate the ethyl acetate extract layer to obtain red rattan Ethyl acetate part extract 12 g.

[0019] (2) The extract from the ethyl acetate part was dissolved in methanol, and subjected to Sephadex LH-20 gel column chromatography, eluted with 90% methanol, and a total of 30 parts of the eluate were collected.

[0020] (3) Fraction 13 is dihydromyricetin, and fraction 25 is myricetin.

Embodiment 2

[0022] (1) Take 500 g of red rattan disc powder, add 90% ethanol to heat and reflux for extraction twice, concentrate the ethanol extract, add distilled water to disperse it, extract twice with ethyl acetate, and concentrate the ethyl acetate extract layer to obtain grass red 54 g of vine ethyl acetate part extract.

[0023] (2) The extract from the ethyl acetate part was dissolved in methanol, and subjected to Sephadex LH-20 gel column chromatography, eluted with 100% methanol, and a total of 30 parts of the eluate were collected.

[0024] (3) Fraction 12 is dihydromyricetin, and fraction 23 is myricetin.

[0025] Compound Structural Identification

[0026] The present invention extracts and separates myricetin and dihydromyricetin from Rhododendron by means of a system solvent method and gel chromatography technology, and obtains their structures through chemical constant determination and spectral analysis, as shown in the attached figure 1 - attached Figure 5 .

[00...

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Abstract

The invention discloses a novel method of extracting and separating myricetin and dihydromyricetin from shuteria pampaniniana. The novel method is characterized by comprising the steps: obtaining an extract through ethanol extraction and ethyl acetate extraction by taking cheap shuteria pampaniniana as a raw material; and carrying out direct gel chromatographic separation on the obtained extract to obtain myricetin and dihydromyricetin. The method provided by the invention is simple to operate, small in loss of samples, high in extraction ratio and suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of medicines, and specifically provides a new method for extracting and preparing myricetin and dihydromyricetin by taking red rattan as a raw material. Background technique [0002] Myricetin and dihydromyricetin have a wide range of pharmacological effects and have great utilization value. Pharmacological activity Previous studies have confirmed that myricetin and dihydromyricetin have various pharmacological effects such as anti-oxidation, anti-tumor, anti-inflammation, anti-alcoholism and liver protection, anti-pathogenic microorganisms, and blood lipid regulation (Hou Xiaolong, Wang Wenqing, Shi Chunyang, et al. 2 Research progress on pharmacological effects of hydromyricetin [J]. Chinese Herbal Medicine, 2015, 46(4): 603-609.). [0003] Grass Red Vine Shuteria pampaniniana Hand.-Mazz. Dried vines with leaves and stems. "Chinese Traditional Medicine Resources Zhiyao" records that Maosubaodou has the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/32C07D311/40
CPCC07D311/30C07D311/32C07D311/40
Inventor 肖世基张茂生
Owner ZUNYI MEDICAL UNIVERSITY