A biological tissue automatic immunohistochemical staining system
An automatic immunization and biological tissue technology, applied in the preparation of test samples, etc., can solve the problems of time-consuming, labor-intensive, and inapplicability.
Active Publication Date: 2020-11-10
SHANXI UNIV
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[0005] The purpose of the present invention is to overcome the shortcomings of the prior art, to provide an automatic immunohistochemical staining system for biological tissue, to solve the problem of manual operation of existing immunohistochemical experiments, which is time-consuming and laborious, and is not suitable for thicker biological tissue immunohistochemical staining technical issues
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[0020] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.
[0021] Such as Figure 1 to Figure 3 As shown, a biological tissue automatic immunohistochemical staining system in this embodiment includes a glass staining tube 1, a primary antibody storage tank 10, a secondary antibody storage tank 11, a PBT buffer solution storage tank 12, and formaldehyde fixative Liquid storage tank 13, liquid inlet pipe 4, liquid outlet pipe 5, several connecting pipes 3 and waste liquid tank 6;
[0022] One end of the glass staining tube 1 is open, and the opening of the glass staining tube 1 is provided with a sealing plug 7, and the sealing plug 7 is provided with a liquid inlet hole 8 and a liquid outlet hole 9;
[0023] In order to prevent the biological tissue from being sucked into the liquid outlet pipe when the liquid is discharged, the bottom side of the glass staining tube 1 is provided with a liquid discha...
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The invention discloses to an automatic biological-tissue immumohistochemical staining system, relates to biological-tissue culture equipment, and solves the technical problem that an existing immumohistochemical experiment adopts manual operation and is time-consuming and labor-consuming and not applicable to the immunohistochemistry of thick biological tissue. The automatic biological-tissue immumohistochemical staining system comprises a glass staining tube, a primary-antibody liquid storage tank, a secondary-antibody liquid storage tank, a PBT buffer solution storage tank, a formalin fixation liquid storage tank, a liquid inlet tube, a liquid outlet tube, a plurality of connecting tubes and a waste liquid tank, wherein one end of the glass staining tube is provided with an opening, a sealing plug is arranged at the opening and provided with a liquid inlet and a liquid outlet, the inner bottom side of the cavity of the glass staining tube is provided with a liquid outlet tube protecting cavity, and the lateral wall of the liquid outlet tube protecting cavity is provided with a plurality of through holes. The automatic biological-tissue immumohistochemical staining system has theadvantages that the specially-made glass staining tube is used, thick biological tissue can be subjected to an immumohistochemical staining experiment in a staining cavity, and the immumohistochemical flow is completed automatically.
Description
technical field [0001] The invention belongs to the technical field of biological tissue culture equipment, in particular to an automatic immunohistochemical staining system for biological tissue. Background technique [0002] Immunohistochemistry is the application of the basic principle of immunology—antigen-antibody reaction, that is, the principle of specific combination of antigen and antibody. Through chemical reaction, the chromogenic agent fluorescein, enzyme, metal ion, and isotope of the labeled antibody are developed to determine the color of the tissue. Intracellular antigenic polypeptides and proteins, for localization, qualitative and quantitative research. [0003] The whole process of immunohistochemical experiment is not only cumbersome, but also requires manual sample addition and liquid replacement at regular intervals, which is time-consuming and labor-intensive, and is prone to shortcomings such as inaccurate sample volume. [0004] An automatic immunoh...
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IPC IPC(8): G01N1/31
CPCG01N1/31
Inventor 邢树平张徐波张建珍马恩波
Owner SHANXI UNIV