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Method for purifying cephalosporin C fermentation broth

A cephalosporin and fermentation liquid technology, applied in organic chemistry and other directions, can solve the problems of separation and purification process influence, high labor intensity, harsh labor environment, etc., and achieve the effect of improving the quality of feed liquid, low labor intensity, and improving yield

Inactive Publication Date: 2018-04-17
YILI CHUANNING BIOTECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Among the existing methods for purifying cephalosporin C fermented liquid, most enterprises adopt plate-type ultrafiltration, plate-and-frame, intermittent ceramic membrane and other filtration methods. The subsequent resin separation and purification process will have a certain impact, which may have a greater impact on the quality of the finished cephalosporin C product
Moreover, the aforementioned method also has disadvantages such as high labor intensity and poor working environment in the production process.

Method used

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  • Method for purifying cephalosporin C fermentation broth
  • Method for purifying cephalosporin C fermentation broth
  • Method for purifying cephalosporin C fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] a. Preparation of reference substance:

[0036] Accurately weighed (24-26 mg) CPC sodium salt reference substance, add an appropriate amount of pH 4.75 sodium acetate buffer solution, dissolve it with ultrasonic waves, transfer it to a 100mL volumetric flask after dissolving, adjust the volume to the mark with buffer solution, and shake well. The preparation of the reference substance should be weighed in duplicate, and the relative average deviation of the response factors after the injection of the two reference substances should be less than 0.5%.

[0037] b. Preparation of sample solution:

[0038] Dilute the original sample solution with sodium acetate buffer solution according to the required dilution factor, so that the diluted concentration is about 200 titer units per milliliter, filter it into the injection vial with a 0.22um disposable needle filter and wait for injection .

[0039] c. Preparation of fermentation broth and acidification solution

[0040] A...

Embodiment 1

[0061] Embodiment 1 The method of the present invention

[0062] The fermentation liquid enters four 160m 3 For the acidification tank, when the liquid level of the acidification tank reaches 30%, the tank is turned on for stirring, and at the same time, the circulating water at -10°C is turned on to keep the temperature of the feed liquid from rising. When the liquid level of the acidification tank reaches 50%, slowly add 3‰ (w / w) oxalic acid, add 20% dilute sulfuric acid to adjust the pH to 3.0-3.2, add 1.5‰ (v / v) formaldehyde, after stirring evenly, Drive into the vibrating sieve through the acidification pump and enter the ceramic membrane raw material tank. When the volume in the tank reaches 30%, turn on the circulating water at -10°C in the tank to keep the temperature of the material liquid from rising. Wait until the liquid level reaches 50m 3When the ceramic membrane raw material pump enters the right-angle filter before the membrane, it enters the ceramic membrane...

Embodiment 2

[0066] Embodiment 2 The method of the present invention

[0067] The fermentation broth enters four 160m 3 For the acidification tank, when the liquid level of the acidification tank reaches 30%, the tank is turned on for stirring, and at the same time, the circulating water at -10°C is turned on to keep the temperature of the feed liquid from rising. When the liquid level of the acidification tank reaches 50%, slowly add 3‰ oxalic acid, add 20% dilute sulfuric acid to adjust the pH to 2.8-3.0, add 1-4‰ formaldehyde, and after stirring evenly, pour it into the vibrating screen through the acidification pump. Enter the ceramic membrane raw material tank, and when the volume in the tank reaches 30%, turn on the circulating water at -10°C in the tank to keep the temperature of the feed liquid from rising. Wait until the liquid level reaches 50m 3 At the same time, the ceramic membrane raw material pump is pumped into the right-angle filter before the membrane, and then enters t...

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Abstract

The invention discloses a method for purifying cephalosporin C fermentation broth. The method comprises the following steps: (1) taking the cephalosporin C fermentation broth, keeping the fermentationbroth at temperature of 5-25 DEG C, adjusting pH to be 2.2-3.2, adding 1-4permillagev / v formaldehyde and 1-5permillagew / w oxalic acid, and evenly mixing the mixture; (2) filtering the mixture by a ceramic membrane; (3) performing ultrafiltration; (4) performing nanofiltration to obtain a filtrate. The method is simple, efficient and easy to operate, problems existing in existing methods are effectively solved, and the method is suitable for popularization and application.

Description

technical field [0001] The invention relates to a method for purifying cephalosporin C fermentation liquid. Background technique [0002] Among the existing methods for purifying cephalosporin C fermented liquid, most enterprises adopt plate-type ultrafiltration, plate-and-frame, intermittent ceramic membrane and other filtration methods. The subsequent resin separation and purification process will have a certain impact, which may have a greater impact on the quality of the cephalosporin C finished product. Moreover, the aforementioned method also has disadvantages such as high labor intensity and poor working environment in the production process. [0003] Therefore, it is necessary to explore a simple and efficient method for purifying cephalosporin C fermentation broth. Contents of the invention [0004] In order to solve the above problems, the invention provides a method for purifying cephalosporin C fermented liquid, comprising the following steps: [0005] (1) T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D501/28C07D501/12
CPCC07D501/12C07D501/28
Inventor 张栗珲刘立苏彦波杨伟邓旭衡刘思川葛均友万阳浴
Owner YILI CHUANNING BIOTECH CO
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