A kind of mesoderm lineage mesenchymal stem cell derived from pluripotent stem cell and preparation method thereof
A technology of pluripotent stem cells and mesenchymal stem cells, applied in the field of mesenchymal stem cells of mesoderm lineage and its preparation, can solve the problems of poor treatment effect, uncontrolled differentiation path of cells, cell heterogeneity, etc., and achieve strong proliferation and immune regulation capacity, enhancing cytology or therapeutic function, addressing source-limited effects
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Embodiment 1
[0040] Example 1 Induction of iPS (induced pluripotent stem cells) to mesoderm mesenchymal stem cells
[0041] Include the following steps:
[0042] 1. Culture of iPS cells
[0043] iPS cells can be conveniently obtained through commercialization or laboratory construction. The present invention focuses on the further cultivation and differentiation operations on the basis of stably established pluripotent stem cell lines. iPS cells can be expanded on a large scale without feeder layers and remain undifferentiated. The culture medium can be selected from the mTeSR series products of STEMCELL company, E8, etc. If it is compatible with the above-mentioned culture medium, it is necessary to coat the culture medium with extracellular matrix, such as Matrigel or laminin. Ensuring high-quality iPS cells is the key to further induction.
[0044] Based on the mTeSR1 and Matrigel culture system, the specific culture operations are as follows:
[0045] 1 Coating of petri dish: Thaw...
Embodiment 2H1
[0068] Example 2 Induction of H1ES embryonic stem cells to mesoderm mesenchymal stem cells
[0069] Include the following steps:
[0070] 1. Culture of H1ES cells
[0071] H1ES cells are readily available commercially. The present invention focuses on the further cultivation and differentiation operations on the basis of stably culturing the H1ES cell line.
[0072] H1ES cells can be expanded on a large scale without feeder layers and remain undifferentiated. The culture medium can be selected from the mTeSR series products of STEMCELL company, E8, etc. If it is compatible with the above-mentioned culture medium, it is necessary to coat the culture medium with extracellular matrix, such as Matrigel or laminin. Ensuring high-quality pluripotent stem cells is the key to further induction.
[0073] Based on the mTeSR1 and Matrigel culture system, the specific culture operations are as follows:
[0074] 1 Coating of petri dish: Thaw Matrigel on ice, dilute Matrigel to 1:20 to...
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