Familial auricular fibrillation gene diagnosis kit

A technology for atrial fibrillation and genetic diagnosis, applied in the field of molecular biology, can solve the problems of inability to meet the requirements of sequencing, low efficiency, and high cost of single nucleotide sequencing, so as to increase the clinical detection rate and accuracy, reduce costs, The effect of improving detection accuracy and stability

Inactive Publication Date: 2018-04-20
FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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  • Application Information

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Problems solved by technology

[0003] As a traditional sequencing technology, Sanger sequencing can only determine one base sequence in one reaction. Although it is accurate, it is inefficient and the cost of single nucleotide sequencing is high.
For the research of compl

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  • Familial auricular fibrillation gene diagnosis kit
  • Familial auricular fibrillation gene diagnosis kit
  • Familial auricular fibrillation gene diagnosis kit

Examples

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Embodiment Construction

[0033] The following examples are only described as examples of the practical application of the present invention, and the practical application of the present invention is not limited to the following examples:

[0034] 1. The present invention is described in further detail and completeness below by means of the examples, but the present invention is not limited to the scope of the examples.

[0035] 2. Use TIANamp Blood DNA Midi Kit (DP332) to extract the genome of the sample to be tested. The volume of elution buffer (TE) is not less than 200 μL, and the OD260 / 280 value reaches 1.8-2.0. Take 2 μL of 25ng / μL DNA as a starting point. start template.

[0036] 3. Quantify the sample with Qubit, and use ddH after quantification 2 O Dilute the g DNA sample to 25 ng / μL.

[0037] 4. Building a database

[0038] 5. DNA fragmentation of genomic samples and addition of adapters and tags

[0039] 6. Add 1.5ml of Ethanol to 4.5ml of TargetCap Stop Solution, and mix thoroughly to m...

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Abstract

The invention discloses a familial auricular fibrillation gene diagnosis kit, and is characterized in that the invention relates to the diagnosis kit for detecting familial auricular fibrillation genes by a large-scale parallel sequencing platform NGS technique. The kit is mainly characterized in that: A, a capture probe is uniquely designed and prepared and aims at all exon fragments of genes ABCC9, GJA5, KCNA5, KCNE2, KCNJ2, KCNQ1, NPPA and SCN5A; B, a connector with a label sequence is uniquely designed; C, a probe sequence is subjected to PCR amplification by universal primers; and D, a uniquely designed target fragment after mixing is subjected to capture operation.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to medical diagnosis and biotechnology, relates to an in vitro diagnostic kit for gene mutation of familial atrial fibrillation, and is a gene diagnosis of familial atrial fibrillation applied to the NGS technology of a new generation sequencing platform Reagent test kit. Background technique [0002] Atrial fibrillation refers to the rapid and ineffective contraction of the atrium. It is one of the most common and serious cardiac arrhythmias in clinical practice. With the increase of age, the prevalence rate increases year by year. Statistics show that its incidence among adults is nearly 1% under the age of 40, about 2.3% over the age of 40, and as high as 5% over the age of 65. The harm of atrial fibrillation to patients is not only palpitations and other symptoms of arrhythmia, but it is easy to induce serious complications, such as heart failure, tachycardia cardiomyopathy, stroke a...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6869
CPCC12Q1/6869C12Q1/6883C12Q2600/156
Inventor 宋雷王继征邹玉宝惠汝太
Owner FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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