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Simple sequence repeat (SSR) molecular markers co-segregated with main-effect quantitative trait loci (QTL) for controlling flax plant height, and application of SSR molecular marker

A technology of molecular markers and co-segregation, applied in the field of molecular biology, can solve problems such as limitations and few genetic markers

Active Publication Date: 2018-04-20
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the plant height of flax is inherited as a quantitative trait, which is dominated by a large number of genes; the effect of each gene on the trait is very small, and these genes have no significant recessive relationship with each other, and their effects are generally cumulative. There are very few genetic markers associated with plant height in flax, limiting their use in molecularly assisted breeding

Method used

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  • Simple sequence repeat (SSR) molecular markers co-segregated with main-effect quantitative trait loci (QTL) for controlling flax plant height, and application of SSR molecular marker
  • Simple sequence repeat (SSR) molecular markers co-segregated with main-effect quantitative trait loci (QTL) for controlling flax plant height, and application of SSR molecular marker
  • Simple sequence repeat (SSR) molecular markers co-segregated with main-effect quantitative trait loci (QTL) for controlling flax plant height, and application of SSR molecular marker

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Experimental program
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Effect test

Embodiment 1

[0050] Example 1 Acquisition of SSR molecular markers co-segregated with high main-effect QTL in control flax strains 1 Materials and methods

[0051] 1 material

[0052] Table 2 124 flax core collections

[0053]

[0054]

[0055] 1.2 Method

[0056] 1.2.1 Basic statistical analysis of plant height traits

[0057] Flax was planted in the Xiangyun Experimental Field of Yunnan Province in 2011 and 2012 respectively. Random block design was adopted, and each resource was planted at 4m 2 In the plot, 20 plants were randomly surveyed in each plot for each resource. The plant height of flax was investigated, and the survey method was collected and sorted according to the "Specifications and Data Standards for the Description and Data Standards of Flax Germplasm Resources" (Wang Yufu et al., 2006).

[0058] The data of plant height traits of 124 flax core germplasms were imported into SPSS18.0 software for descriptive statistical analysis, and the maximum, minimum, average...

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Abstract

The invention discloses simple sequence repeat (SSR) molecular markers co-segregated with main-effect quantitative trait loci (QTL) for controlling flax plant heights. The SSR molecular markers are selected from at least one of GD264, GD282 and GD201. The three SSR molecular markers provided by the invention show a significant correlation with the plant heights in 124 flax core germplasm resources, and are stably existing plant height main effect loci; primers, which are provided by the invention and used for amplifying the SSR molecular markers, are directly used for screening excellent germplasm, and realizing gene positioning and cloning as well as molecular marker assisted breeding; therefore, the SSR molecular markers have great significance in cultivation or research of high-yield fiber flax varieties. Furthermore, the SSR molecular markers provide beneficial reference of molecular marker breeding.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to an SSR molecular marker co-separated with a high main effect QTL controlling flax strains and its application. Background technique [0002] Molecular markers are genetic markers based on the polymorphism of the genetic material nucleic acid of an organism. They have the characteristics of abundant quantity, stable genetics, no restriction on gene expression, and easy operation. Molecular markers can be used to directly detect genome Genetic Variation. At present, a variety of molecular marker techniques have been applied in the basic research of flax, including RAPD, RFLP, AFLP, SSR, SRAP, ISSR, IRAP and SNPs. However, the number of markers obtained in the above studies is still small, and the most widely used of these markers is the SSR marker. SSR markers have the advantages of stable amplification, high specificity, co-dominance, and relatively low development costs. [000...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 邓欣
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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