A method for isolating formic acid-producing and acetogenic bacteria that degrade butyric acid

Abstract

The invention discloses a separation method of butyric acid-degrading formic acid producing acetogens. In a stage of enrichment culture, formic acid and butyric acid are adopted as a co-substrate foracclimatizing microorganisms, ecological functions of the formic acid producing acetogens (FPAs) are enhanced indirectly by enriching formic acid type methanogens, and effective enrichment of FPAs isrealized. In a stage of isolated culture, a butyric acid metabolic intermediate crotonic acid serves as a substrate, and sodium 2-bromoethanesulfonate (2-BES) is added to inhibit methanogenesis so asto avoid consumption of formic acid by methanogens; the formic acid in a product can be effectively detected so as to understand the enrichment of formic acid producing bacteria and the formic acid producing ability of the formic acid producing bacteria more conveniently and visually. By screening the microorganisms with formic acid producing ability, the probability of successfully separating andculturing the butyric acid degrading formic acid producing acetogens in later period is greatly increased.

Description

technical field [0001] The invention relates to the field of sewage biological treatment and its reuse, in particular to a method for separating formic and acetogenic bacteria that degrade butyric acid. Background technique [0002] Anaerobic biological treatment technology has the advantages of combining pollution control and production capacity, and has become an important wastewater treatment technology at home and abroad. During the start-up process of the anaerobic reactor, due to the rapid growth and metabolism of acidogenic fermentation microorganisms, organic matter is rapidly hydrolyzed and acidified to produce a large amount of volatile fatty acids (VFAs) such as propionic acid and butyric acid. When VFAs are converted, the accumulation of VFAs will not only inhibit the subsequent methanogenesis reaction, but also feedback and inhibit the continued hydrolysis of the remaining organic matter, resulting in "rancidity" of the reactor. Acid accumulation has become a b...

AI Technical Summary

Problems solved by technology

The existing bacteria separation method does not specifically target the enrichment of formic acid-producing and acetogenic bacteria in the early stage of enrichment culture, and the formic acid in the product is easily consumed by methanogenic bacteria, which is difficult to detect, so it is impossible to detect formic acid-producing and acetogenic bacteria. For effective enrichment, it is also impossible to detect the enrichment of formic acid-producing and acetogenic bacteria, resulting in difficulties in the separation of formic and acetogenic bacteria