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Three-dimensional cell gradient mechanics loading experiment platform based on tissue specific shape

A technology of three-dimensional cells and specific shapes, which is applied in the field of mechanical stimulation of cells and tissues, can solve the problems that mechanical gradients and mechanical stimulation cannot be simulated, and achieve the effects of adjustable mechanical gradient, controllable size and convenient use

Inactive Publication Date: 2018-05-04
XIAN INT UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a three-dimensional cell gradient mechanical loading experimental platform based on the specific shape of the tissue, which solves the problem of existing bioreactors and mechanical loading equipment and instruments mainly by adjusting the amplitude, frequency, duration and strength of mechanical stress. Realize the gradient mechanical loading experiment of dimensional cells, and these instruments can only achieve uniform mechanical stimulation, and cannot simulate the defect of mechanical gradient around the body tissue

Method used

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  • Three-dimensional cell gradient mechanics loading experiment platform based on tissue specific shape
  • Three-dimensional cell gradient mechanics loading experiment platform based on tissue specific shape
  • Three-dimensional cell gradient mechanics loading experiment platform based on tissue specific shape

Examples

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preparation example Construction

[0040] The preparation of the three-dimensional tissue module 3 encapsulating cells is firstly to mix the biological material and the cells, and then inject the formed mixture into a mold with a specific geometric configuration prepared from organic or inorganic materials, and the specific geometric configuration The type of die is a geometric configuration with a mechanical gradient.

[0041] The geometric configuration of the mechanical gradient is a conical structure or a terraced structure.

[0042] The shape of the three-dimensional tissue module 3 encapsulating the cells is a geometric configuration with a mechanical gradient, preferably a frustoconical structure or a terraced structure.

[0043] The three-dimensional tissue module 3 encapsulating the cells is respectively connected to the first splint 1 and the second splint 2 through 502 glue.

[0044] The two splints are respectively provided with four passage holes corresponding to each other. The first splint 1 and...

Embodiment 1

[0052] refer to Figure 1a and Figure 1b , use computer to design two rectangles (the length of the rectangle is 25mm, the width is 15mm, and the thickness is 5mm) as the upper and lower splints of the three-dimensional cell gradient mechanical loading mold. The mechanical loading device is obtained by cutting the organic material PMMA. Four through holes with the same diameter as the threaded column b are drilled at both ends of the two PMMA splints, and the diameter of the threaded column b is 1.5 mm.

[0053] A cell-wrapped three-dimensional tissue module 3 is sandwiched between the two splints, and the contact parts between the cell-wrapped three-dimensional tissue module 3 and the two splints are bonded with 502 glue. The two splints are arranged in parallel, and the two ends of the two splints are provided with through holes with a diameter of 1.5 mm. The two through holes corresponding to the positions of the two splints pass through a threaded column a, and the outer ...

Embodiment 2

[0057] refer to Figure 4 , a truncated circular three-dimensional cell mechanical gradient dynamic loading platform, comprising a mold composed of two splints 1, 2, a cell-encapsulated three-dimensional tissue module 3 is sandwiched between the two splints 1, 2, a cell-encapsulated three-dimensional tissue module The contact parts between the module 3 and the splints 1 and 2 are connected with 502 glue, and the two ends of the two splints 1 and 2 are respectively connected to the control clips 6 on the one-dimensional mobile platforms 4 and 5, and the four control clips 6 are respectively connected to the first A motor 8, a second motor 9, a third motor 10 and a fourth motor 11 are connected. The computer can control the four motors to move on the one-dimensional mobile platform, and the displacement is transmitted to the two splints 1 and 2 through the four control clips 6 to generate strain gradients.

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Abstract

The invention provides a three-dimensional cell gradient mechanics loading experiment platform based on a tissue specific shape. The three-dimensional cell gradient mechanics loading experiment platform comprises a first clamping plate and a second clamping plate, which are arranged in a mutual parallel manner, wherein a three-dimensional tissue module for covering cells or a three-dimensional tissue module array is arranged between the first clamping plate and the second clamping plate; the three-dimensional tissue module for covering the cells is a geometrical configuration with a mechanicsgradient; meanwhile, the three-dimensional tissue module for covering the cells is fixedly connected with the first clamping plate and the second clamping plate respectively; the first clamping plateis movably connected with the second clamping plate; a distance between the two clamping plates is adjusted so that a mechanical stretching or compression effect of the three-dimensional tissue moduleis realized, and furthermore, the three-dimensional tissue module is deformed after being stretched or compressed; gradient strain is formed in the three-dimensional tissue module so that cell behaviors are influenced; a high-flux three-dimensional cell gradient mechanics loading device and an experiment platform can be formed through an array design of a loading device; the mechanics loading experiment platform provided by the invention has the advantages of low cost, simplicity in preparation, convenience for utilization, controllable size, adjustable mechanics gradient and the like.

Description

technical field [0001] The invention relates to the technical field of cell and tissue mechanical stimulation research, in particular to a three-dimensional cell gradient mechanical loading experiment platform based on a specific shape of tissue. Background technique [0002] Somatic cells are in a complex cellular microenvironment, and the dynamic balance of the microenvironment is an important condition for maintaining the normal behavior and function of cells. Mechanical stimuli in the microenvironment play an important role in regulating cell behavior, and participate in the regulation of proliferation, migration and differentiation of different cells in development, inflammation, wound healing and cancer. More and more studies have found that various mechanical signals in the microenvironment are not always uniform, and in many cases exist in the form of gradients and participate in the regulation of cell behavior, playing a complex role in the control of body developme...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M1/00C12Q1/02
CPCC12M35/04C12M41/00G01N33/5005
Inventor 王琳董明清张晓峰何楠李娜周宝珍许宁侠徐靖
Owner XIAN INT UNIV
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