Fast Fluorescence Lifetime Imaging Method Based on Single Photon Avalanche Diode Detector
A technology of single-photon avalanche and fluorescence lifetime, which is applied in the direction of instruments, can solve the problems of unrepresentative data, small optimal detection range, and slow imaging speed, so as to reduce the number of laser excitations, increase the amount of information collected, and increase data The effect of collection volume
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[0029] The present invention is described in further detail now in conjunction with accompanying drawing.
[0030] The invention uses single-photon avalanche diodes to detect fluorescent photons and record related information, so as to calculate the fluorescent lifetime of substances. This method uses discrete and aliasing multiplexed detection windows for specific sampling of fluorescence decay, and then the fluorescence decay curve of the substance (such as figure 2 Shown) fit out, so as to calculate the fluorescence lifetime of the substance, the fluorescence lifetime measurement structure is as follows figure 1 shown. The process of detecting the fluorescence decay of the window is as follows: image 3 shown.
[0031] Pixel unit modules such as Figure 5 As shown, the module includes the SPAD and its control circuit for controlling its working state, the quenching circuit for quenching the high avalanche current of the SPAD, the avalanche pulse gating circuit, the ava...
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