Hematocrit measurement method and blood detection method

A technology for hematocrit and blood testing, which is used in measuring devices, biological testing, material inspection products, etc.

Active Publication Date: 2020-06-19
IND TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Allow whole blood sample to enter reaction zone

Method used

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  • Hematocrit measurement method and blood detection method
  • Hematocrit measurement method and blood detection method
  • Hematocrit measurement method and blood detection method

Examples

Experimental program
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Effect test

experiment example 1

[0053] For whole blood samples with different hematocrit ratios and different blood glucose levels, proceed as follows Figure 1A Step S100 , step S102 and step S104 are shown. Please refer to Figure 2 to Figure 4 , the initial voltage E of the square wave voltage V1 of Experimental Example 1 init is 0V, the fixed voltage amplitude Amp is 0.01V, and the increasing voltage E incr is 0.01V, and the frequency F is 300Hz. In addition, the interval between the start time T0 when the whole blood sample enters the first reaction zone 202 of the test strip 200 and the start time T1 when a set of square wave voltage V1 is applied to the first electrode 204 is 0.5 seconds.

experiment example 2

[0064] For the whole blood sample A to the whole blood sample E known to have different hematocrit ratio, carry out as follows Figure 1A Shown are step S100, step S102 and step S104. Whole blood sample A has a known hematocrit of 20%, whole blood sample B has a known hematocrit of 31%, whole blood sample C has a known hematocrit of 40%, whole blood sample D has a known hematocrit of The volume ratio is 51% and the known hematocrit of whole blood sample E is 60%. Please refer to Figure 2 to Figure 4 , the initial voltage E of the square wave voltage V1 of Experimental Example 2 init is 0.15V, the fixed voltage amplitude Amp is 0.17V, and the increasing voltage E incr is 0.04V, and the frequency F is 750Hz. In addition, the interval between the start time T0 when the whole blood sample enters the first reaction zone 202 of the test strip 200 and the start time T1 when a set of square wave voltage V1 is applied to the first electrode 204 is 5 seconds.

experiment example 3

[0073] In Experimental Example 3, the following steps were carried out sequentially under different humidity environments: Figure 5AStep S100 , step S102 and step S504 are shown. In Experimental Example 3, multiple sets of square wave voltage VS were continuously applied to the first electrode by square wave voltammetry, and the interval between two adjacent sets of square wave voltage VS was 1 second. Next, the hematocrit of the whole blood sample is estimated from the feedback value of the square wave voltage VS of the third group. Before performing the above steps, the machine for applying voltage to the first electrode 204 of the detection test strip 200 is placed in environments with respective humidity of 31%, 60% and 90% and equilibrated for 30 minutes. Then, the above step S100 , step S102 and step S504 are performed for whole blood samples with different hematocrit ratios (hematocrit ratios are 20%, 31%, 40%, 50% and 59%, respectively). In step S504, the initial vo...

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Abstract

The invention provides a method for measuring hematocrit and a method for detecting blood. The measuring method of the hematocrit ratio includes the following steps. Test strips are provided. The test strip includes a reaction area and a pair of electrodes arranged in the reaction area. Allow the whole blood sample to enter the reaction zone. After the whole blood sample enters the reaction area, a set of square wave voltages is applied to the above pair of electrodes by square wave voltammetry to obtain feedback values ​​related to the hematocrit ratio. The difference between the initial time point when the whole blood sample enters the reaction zone and the initial time point when a set of square wave voltages are applied is 0.1 to 200 seconds. Estimate hematocrit volume ratio based on the feedback value.

Description

technical field [0001] The present invention relates to a method for measuring hematocrit ratio and a blood detection method, and relates to a method for measuring hematocrit ratio and a blood detection method for whole blood samples. Background technique [0002] Hematocrit (Hct) mainly refers to the proportion of blood cells (mainly red blood cells) that are precipitated after centrifugation of whole blood with anticoagulant added in the whole blood. For clinical laboratory testing machines, plasma is used as the sample for testing. In other words, the blood is first centrifuged, and the resulting plasma is used for testing. In this way, the difference in hematocrit ratio will not interfere with the test results. [0003] However, for point of care testing (POCT) or testing machines that patients can operate (over the counter, OTC), in order to reduce the time and cost required for testing, it is generally used full Blood samples are directly measured. In other words, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/05G01N27/27
CPCG01N15/05G01N27/27G01N27/3274G01N33/48785G01N33/49G01N27/02G01N27/42G01N27/48G01R27/22
Inventor 陈筑瑄颜钰芳董易庭林芬菲袁亦云谢文彬
Owner IND TECH RES INST
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