Three-dimensional single molecule tracking method and device

A tracking device and three-dimensional single technology, applied in the field of computational microscopy, can solve the problems of incapable of rapid dynamic process imaging, limited application range, long acquisition time, etc., achieve high-precision three-dimensional single-molecule positioning, improve accuracy, and simple structure Effect

Active Publication Date: 2018-05-08
TSINGHUA UNIV
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  • Abstract
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Problems solved by technology

This technique cannot locate samples that are far away from the focus, and is greatly affected by noise, or it can be excited by layer scanning
This method can accurately obtain the three...

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  • Three-dimensional single molecule tracking method and device
  • Three-dimensional single molecule tracking method and device
  • Three-dimensional single molecule tracking method and device

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Embodiment Construction

[0030] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0031] The following describes the three-dimensional single-molecule tracking method and device according to the embodiments of the present invention with reference to the drawings. First, the three-dimensional single-molecule tracking method according to the embodiments of the present invention will be described with reference to the drawings.

[0032] figure 1 is a flow chart of the three-dimensional single-molecule tracking method of the embodiment of the present invention.

[0033] Such as figure 1 As shown, the three-dimen...

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Abstract

The invention discloses a three-dimensional single molecule tracking method and device, wherein the method comprises: taking the image plane of a fluorescence microscope as a relay image plane so as to place a micro lens array at the position of the relay image plane; setting parameters so as to enable cooperation with an objective lens with a high numerical aperture in order to acquire the three-dimensional light field information of a sample on a camera surface; processing the acquired image by using a compressed sensing algorithm, using a pre-calculated light field feature image as the sparse basis and using the L-1 norm minimization constraint to obtain the optimal expression under the sparse basis, and obtaining the three-dimensional location information of the sample. The method canachieve high-speed and high-precision three-dimensional single-molecule positioning, significantly improves the imaging accuracy, greatly improves the performance, and is low in transformation cost, simple in structure, and easy to implement.

Description

technical field [0001] The invention relates to the technical field of computational microscopy, in particular to a three-dimensional single-molecule tracking method and device. Background technique [0002] Fluorescence microscopy plays a very important role in life science research. On the cell scale, its main components are solutions, which are not visible by itself. Therefore, staining or fluorescent labeling methods are often used in its research. Compared with staining methods, fluorescent labeling technology is more convenient to control and operate, and is more conducive to distinguishing regions of interest. The method of fluorescence microscopy mainly uses fluorescent dyes or fluorescent proteins to label organisms, uses short-wavelength lasers for excitation and irradiation, and uses camera sensors at the acquisition end to collect long-wavelength emission signals. [0003] The commonly used commercial microscopes in the related art can realize fluorescence micr...

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Application Information

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IPC IPC(8): G06T7/246
CPCG06T2207/10056G06T7/246
Inventor 戴琼海席鹏陈星晔张昊谢浩
Owner TSINGHUA UNIV
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