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A method for collecting oral secretions of small Lepidoptera larvae

A technology for Lepidoptera larvae and oral secretions, applied in animal husbandry and other fields, can solve the problems of slow research progress and inability to obtain oral secretions, and achieve the effects of high collection efficiency, easy operation, and low cost

Inactive Publication Date: 2020-02-14
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for smaller Lepidoptera larvae, such as diamondback moth, none of the above methods can obtain a large amount of oral secretions
At present, there is still a lack of effective methods for collecting small Lepidoptera larvae, which has resulted in slow progress in the research on the mechanism of interaction between small Lepidoptera pests and plants

Method used

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  • A method for collecting oral secretions of small Lepidoptera larvae
  • A method for collecting oral secretions of small Lepidoptera larvae
  • A method for collecting oral secretions of small Lepidoptera larvae

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Embodiment Construction

[0020] In order to make the above-mentioned features and advantages of the present invention more comprehensible, the following specific embodiments are described in detail with reference to the accompanying drawings.

[0021] Such as Figure 1~3 As shown, a method for collecting oral secretions of small Lepidoptera larvae comprises the following steps:

[0022] (1) Use the roller-type pressing device 1 to roll back and forward the middle and front part of the small Lepidoptera larvae 2;

[0023] (2) When the small Lepidoptera larvae 2 spit out the oral secretion 4, use the pipette 3 to collect the oral secretion 4;

[0024] (3) Finally, blow the oral secretion 4 in the pipette 3 into the collection container 6 with the blowing device 5 .

[0025] In the embodiment of the present invention, the roller pressing device 1 includes a handle 101, the front end of the handle 101 is provided with a connecting shaft 102, and the connecting shaft 102 is covered with a tube body 103 f...

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Abstract

The invention relates to a method for collecting oral secreta of minitype lepidopterous larvae. The method is characterized by comprising the following steps of 1, using a roller-type pressing deviceto roll the middle front part of minitype lepidopterous larvae to reciprocate back to forth; 2, after the minitype lepidopterous larvae spit the oral secreta, using a liquid-moving device to collect the oral secreta; 3, finally using a blast device to blow the oral secreta in the liquid-moving device into a collecting container. The collecting method is easy to operate, material are easy to collect, the cost is low, and the collecting efficiency is high, only one man is needed for the operation of collecting the oral secreta of the minitype lepidopterous larvae, and the application prospect isgood.

Description

technical field [0001] The invention relates to a method for collecting oral secretions of small Lepidoptera larvae. Background technique [0002] Oral secretions of Lepidoptera insects, also known as regurgitation, often contain insect saliva, midgut digestive juice and other components. When Lepidoptera larvae feed on plants, elicitors in their oral secretions can trigger plant defense responses. Obtaining the oral secretions of Lepidoptera larvae is an important prerequisite for in-depth study of the relationship between insects and plants and to elucidate the mechanism of insect-induced plant defense responses. [0003] At present, the main methods to obtain oral secretions from lepidopteran insect larvae are (1) press the body of the larvae to obtain oral secretions; (2) freeze the larvae for a few minutes and then place them at room temperature until they recover to spit out oral secretions; (3) knock The head of the insect, causing the insect to spit out oral secret...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 陈文滨尤民生张帅琪毛军
Owner FUJIAN AGRI & FORESTRY UNIV