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Method for determining total content of oleanolic acid in herba verbenae

A technology of oleanolic acid and verbena, which is applied in the field of chemical detection, can solve the problems of deviation of quantitative analysis results, inability to accurately measure, and difficulty in separation and measurement, and achieve the effects of reducing surface tension, increasing capacity, and increasing diffusion

Inactive Publication Date: 2018-05-11
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Oleanolic acid is an isomer with very similar structures and properties. It often coexists and coexists in natural plants, and its separation and determination are difficult.
The 2005 edition of "Chinese Pharmacopoeia" (Part One) adopts thin-layer scanning chromatography to measure the content of ursolic acid in Verbena. Deviation, and can not accurately determine the content of another active ingredient - oleanolic acid

Method used

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  • Method for determining total content of oleanolic acid in herba verbenae
  • Method for determining total content of oleanolic acid in herba verbenae
  • Method for determining total content of oleanolic acid in herba verbenae

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Embodiment 1

[0024] 1. Instruments and reagents

[0025] 1.1 Instruments:

[0026] Electronic Analytical Balance (XA205DU), ASE350 Fast Solvent Extractor (American DIONEX Company), High Performance Liquid Chromatography (1290)

[0027] 1.2 Reagents (unless otherwise specified, all reagents used in this experiment are of analytical grade):

[0028] Water: Class I water in accordance with GB / T 6682;

[0029] 75% ethanol (CH 4 O): chromatographically pure (for liquid chromatography);

[0030] Ethanol (C 2 h 5 OH): analytically pure.

[0031] 2 methods

[0032] 2.1 Preparation of reference solution:

[0033] Take an appropriate amount of oleanolic acid reference substance and ursolic acid reference substance, weigh them accurately, add 75% ethanol to make a mixed solution containing 50 μg of oleanolic acid and 0.1 mg of ursolic acid per 1 ml, and obtain it.

[0034] 2.2 Preparation of the test solution

[0035] 2.2.1 "Chinese Pharmacopoeia" 2015 Edition Part I Preparation method for ...

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Abstract

The invention discloses a method for determining the total content of oleanolic acid in herba verbenae, belongs to the field of chemical detection and aims to provide an ASE-HPLC (accelerated solventextraction-high performance liquid chromatography) method for determining the content of oleanolic acid in the herba verbenae. The method is simple to operate, high in sensitivity and good in stability. According to the method, the crushed herba verbenae is extracted with 75% ethanol by ASE, and 75% ethanol extract is collected; the content of oleanolic acid in the herba verbenae is determined with HPLC, wherein a used chromatographic column is Thermo Acclaim C30. The method can be used for quality control and evaluation of the herba verbenae.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to a method for measuring the total content of oleanolic acid in verbena. Background technique [0002] Verbena is the dry above-ground part of the Verbenaceae plant. It is cool in nature, bitter in taste, and non-toxic; it has the effects of clearing away heat and detoxification, breaking blood and stimulating menstruation, promoting blood circulation and dispelling blood stasis, diuresis and detumescence. Studies have shown that Verbena has obvious anti-inflammatory, anti-early pregnancy and anti-human choriocarcinoma effects. Further research shows that its anti-cancer active ingredient is oleanolic acid. [0003] Oleanolic acid is an isomer with very similar structures and properties. It often coexists and coexists in natural plants, and its separation and determination are difficult. The 2005 edition of "Chinese Pharmacopoeia" (Part One) adopts thin-layer scanning chromatogr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/74G01N30/06G01N30/86
Inventor 陈学松钟水桥梁峰
Owner WUZHOU INST FOR FOOD & DRUG CONTROL