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Cyclic RNA molecular marker for predicting prognosis and death risk of colorectal cancer and application of cyclic RNA molecular marker

A molecular marker, colorectal cancer technology, applied in the field of biomedicine to achieve the effect of prolonging survival and improving accuracy

Active Publication Date: 2018-05-15
SUN YAT SEN UNIV CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Patents CN201510160436.1, CN201510160437.6, CN201510160145.2, CN201610518307.X and CN201610533247.9 all disclose circular RNA molecules related to colorectal cancer, but they are all detected by a single circular RNA molecule, and all target It is the prediction or diagnosis of colorectal cancer; so far, there is no report on the use of circular RNA molecules to predict the prognosis and death risk of patients with colorectal cancer in the middle stage

Method used

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  • Cyclic RNA molecular marker for predicting prognosis and death risk of colorectal cancer and application of cyclic RNA molecular marker
  • Cyclic RNA molecular marker for predicting prognosis and death risk of colorectal cancer and application of cyclic RNA molecular marker
  • Cyclic RNA molecular marker for predicting prognosis and death risk of colorectal cancer and application of cyclic RNA molecular marker

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Embodiment 1

[0028] Example 1 Circular RNA molecules used to predict the prognosis and death risk of colorectal cancer and the establishment of a prognostic risk model

[0029] 1. Screening of circular RNA molecules and establishment of prognostic risk models

[0030] (1) Select 20 cases of mid-term colorectal cancer tumor tissues and 20 cases of normal tissues, among which 20 cases of mid-stage colorectal cancer patient tissues were divided into 10 cases of recurrent patient tissues and 10 cases of non-recurrence patient tissues, and the TRIZOL method was used to extract the RNA;

[0031] (2) Use the high-depth sequencing method of RNA-seq to detect the expression of all RNAs in the tissues of step (1), identify existing and newly discovered circular RNAs, and screen for high expression in tumor tissues of patients with colorectal cancer in the middle stage compared with normal tissues Circular RNA, and the circular RNA highly expressed in tissues of patients with mid-term colorectal can...

Embodiment 2

[0053] Example 2 Kit for Predicting Medium-Term Colorectal Cancer Prognosis and Death Risk

[0054] A kit for predicting the prognosis and risk of death of colorectal cancer in the mid-term, the kit includes the following fluorescent quantitative PCR primers for detecting 4 circular RNA molecules:

[0055]

[0056] The fluorescent quantitative PCR amplification reaction system of the circular RNA molecule is: use the kit of the fluorescent quantitative PCR of Promega ( qPCR Master Mix, product number: A6001), the PCR reaction detection system is 10ul.

[0057] The reaction system is as follows:

[0058]

[0059]

[0060] The procedure of the fluorescent quantitative PCR amplification reaction system for the circular RNA molecule is as follows: After configuring the components required for the PCR reaction, preheat the PCR instrument at 95°C for 2 minutes to fully denature the template DNA, and then enter the amplification cycle . In each cycle, the template was de...

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Abstract

The invention discloses a cyclic RNA molecular marker for predicting prognosis and death risk of colorectal cancer and application of the cyclic RNA molecular marker. The cyclic RNA molecular marker is prepared from circRNA13452, hsa_circ_0008039, hsa_circ_0079480 and hsa_circ_0087391; the cDNA sequences of the cyclic RNA molecular marker are as shown in SEQ ID NO:1 to 4 sequentially. By detectingthe expression levels of the four cyclic RNA molecules in tissues of medium-term colorectal cancer patients and using corresponding predictive models, the aim of predicting the prognosis and death risk of the patients is realized; the accuracy of predicting the prognosis and death risk by using tissue specimens of the colorectal cancer patients is improved; the cyclic RNA molecular marker has great significance for further diagnosis and treatment scheme and prolonging of lifetime of the patients.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and more specifically discloses a circular RNA molecular marker for predicting the prognosis and death risk of colorectal cancer and its application. Background technique [0002] Colorectal cancer (CRC) is one of the top ten malignant tumors in the world and one of the most common malignant tumors in my country. In my country, the morbidity and mortality of colorectal cancer rank among the forefront of malignant tumors. According to a nationwide survey of causes of death, colorectal cancer ranks fifth and fourth among male and female deaths due to malignant tumors. Because the early symptoms of colorectal cancer are not obvious, and most patients lack the awareness of census, they often go to the hospital for treatment when symptoms appear. At this time, most of them are advanced colorectal cancer. However, 50% of patients will still experience recurrence and metastasis if combined with ad...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 徐瑞华鞠怀强赵齐李婷
Owner SUN YAT SEN UNIV CANCER CENT
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