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2,4-dinitrotoluene sulfonate degrading bacterial strain and application thereof

A technology of dinitrotoluene and bacterial strains, applied in the field of environmental biology

Active Publication Date: 2018-05-18
BEIJING INST OF COLLABORATIVE INNOVATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No studies have indicated that microorganisms that can degrade dinitrotoluenesulfonate have been isolated in soil

Method used

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  • 2,4-dinitrotoluene sulfonate degrading bacterial strain and application thereof
  • 2,4-dinitrotoluene sulfonate degrading bacterial strain and application thereof
  • 2,4-dinitrotoluene sulfonate degrading bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Simulation TNT red water polluted soil remediation test

[0046]Take uncontaminated soil, air-dry and grind, pass through a 1mm sieve, and set aside. A certain amount of 2,4-DNT-3-SA and 2,4-DNT-5-SA was weighed and dissolved in acetone. In the fume hood, the acetone solution of the two sulfonates was evenly sprayed into the above soil and stirred evenly. The soil concentration was made approximately 500 mg / kg each of 2,4-DNT-3-SA and 2,4-DNT-5-SA; it was allowed to air dry naturally in a fume hood for 2 days. Pick a single colony in sterilized LB liquid medium, seal it with a gas-permeable sealing film, place the Erlenmeyer flask in a shaker at 120 rpm, and activate at 30°C for 24 hours. Weigh 20g of the above soil, add 10% inoculum amount of Bacillus sp.X1 activated bacteria solution with OD600=1, adjust the water-to-soil ratio to 2:5 with inorganic salt liquid medium, and seal with air-permeable sealing film. All Erlenmeyer flasks were placed in a co...

Embodiment 2

[0047] Example 2: The degradation effect of Bacillus sp.X1 on sulfonate at different pH values

[0048] Take uncontaminated soil, air-dry and grind, pass through a 1mm sieve, and set aside. A certain amount of 2,4-DNT-3-SA and 2,4-DNT-5-SA was weighed and dissolved in acetone. In the fume hood, the acetone solution of the two sulfonates was evenly sprayed into the above soil and stirred evenly. The soil concentration was made approximately 500 mg / kg each of 2,4-DNT-3-SA and 2,4-DNT-5-SA; it was allowed to air dry naturally in a fume hood for 2 days. Weigh 20 g of the above soil into a conical flask, and use dilute H 2 SO 4 and NaOH to adjust the pH of the soil to be 3, 5, 7, 9, 11 respectively, add the bacterium liquid activated by the Bacillus sp.X1 of OD600=1 according to the inoculum size of 10%, and adjust the water-soil ratio with the inorganic salt liquid medium to be 2: 5. Seal with breathable parafilm. All the Erlenmeyer flasks were placed in a constant temperatur...

Embodiment 3

[0049] Embodiment 3: Bacillus sp.X1 is to the degradation effect of sulfonate at different temperatures

[0050] Take uncontaminated soil, air-dry and grind, pass through a 1mm sieve, and set aside. A certain amount of 2,4-DNT-3-SA and 2,4-DNT-5-SA was weighed and dissolved in acetone. In the fume hood, the acetone solution of the two sulfonates was evenly sprayed into the above soil and stirred evenly. The soil concentration was made approximately 500 mg / kg each of 2,4-DNT-3-SA and 2,4-DNT-5-SA; it was allowed to air dry naturally in a fume hood for 2 days. Weigh 20 g of the above soil into an Erlenmeyer flask, add Bacillussp.X1 activated bacterial solution with OD600=1 according to 10% inoculation amount, adjust the water-to-soil ratio to 2:5 with an inorganic salt liquid medium, and seal it with a gas-permeable sealing film. The Erlenmeyer flasks were placed in constant temperature incubators at 15°C, 20°C, 25°C, 30°C, 35°C, and 40°C for a period of time, and samples were...

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Abstract

The invention discloses a method for separating bacteria from TNT (trinitrotoluene) red water polluted soil to degrade main pollutants such as 2,4-dinitrotoluene-3-sulfonate (2,4-DNT-3-SA) and 2,4-dinitrotoluene-5-sulfonate (2,4-DNT-5-SA) in red water. The TNT red water polluted soil is collected, bacteria in the soil are subjected to enrichment, separation and purification cultivation, an efficient degrading bacterial strain X1 is screened, the name of the preservation unit is China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.14587. The degrading bacterial strain has very good degrading effect on the 2,4-DNT-3-SA and the 2,4-DNT-5-SA in the soil, and the degradation rate of the 2,4-DNT-3-SA and the 2,4-DNT-5-SA of 500 mg / kg reaches to100 percent.

Description

technical field [0001] The invention belongs to the field of environmental biology, and in particular relates to a TNT red water polluted soil degradation bacterial strain and its application in TNT red water and TNT red water polluted soil restoration. Background technique [0002] 2,4,6-Trinitrotoluene (TNT) is a military explosive that has been used for over 50 years. In the production process, a large amount of wastewater called red water is produced. TNT red water contains more than 30 kinds of nitroaromatic compounds including dinitrotoluene sulfonate (DNTS), TNT, dinitrotoluene (DNT), nitrotoluene (MNT) and other nitrobenzene derivatives, the main pollutant is 2,4-dinitrotoluene-3-sodium sulfonate (2,4-DNT-3-SO 3 Na) and 2,4-dinitrotoluene-5-sodium sulfonate (2,4-DNT-5-SO 3 Na). TNT red water has complex components, high chroma, high COD, strong toxicity, and is difficult to biodegrade. It is very harmful to the ecological environment and human health. Therefore, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/07C02F101/38C02F103/36
CPCB09C1/10C02F3/34C02F2101/38C02F2103/36C12N1/205C12R2001/07
Inventor 叶正芳徐文杰李智林
Owner BEIJING INST OF COLLABORATIVE INNOVATION
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