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A method for tissue culture and rapid propagation of Acer palmatum

A technology of Acer palmatum red division and tissue culture rapid propagation, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problem of not being able to quickly and efficiently meet the needs of scientific research and large-scale production, and the excellent traits cannot be well The maintenance of seedlings and the large variability of seed propagation plants can meet the needs of large-scale industrial seedling cultivation and industrialization development, with low cost and short cultivation time.

Active Publication Date: 2020-11-24
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acer palmatum grows slowly and its branches are soft. At present, the main propagation methods are grafting and seed propagation. The plant variability of seed propagation is large, and excellent traits cannot be well maintained; grafting propagation is affected by seasons and female scion. impact, unable to quickly and efficiently meet the needs of scientific research and mass production

Method used

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  • A method for tissue culture and rapid propagation of Acer palmatum
  • A method for tissue culture and rapid propagation of Acer palmatum
  • A method for tissue culture and rapid propagation of Acer palmatum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The method for tissue culture and rapid propagation of Acer palmatum red division, including

[0033] Pretreatment: Take the current-year-old branches of Acer palmatum, remove the leaves, cut into budded stem segments with 1 to 2 buds, perform sterilization and cleaning treatment, and obtain sterilized explants;

[0034] Initiating culture: inoculate the sterilized explants in the initiating medium for initiating culture to obtain elongated axillary buds; the initiating medium includes: WPM salts and H organic, supplemented with 0.005-0.01mg / LTDZ, 0.05~0.1mg / LNAA, 0.1~0.4%PPM;

[0035] Proliferation culture: inoculate the axillary buds in the proliferation medium for proliferation culture to obtain sterile sprouts; the proliferation medium includes: WPM salts and H organic, supplemented with 0.15-0.25mg / LCPPU, 0.02-0.06mg / L2 ,4-D;

[0036] Rooting culture: after removing the callus from the base of the sterile sprouts, inoculate them on the rooting medium for rooting cu...

Embodiment 2

[0041] Effects of Components in Initiating Medium on Initiating Culture of Axillary Buds

[0042] Pretreatment: Take the branches of Acer palmatum in the same year, remove the leaves, cut into budded stems with 1-2 buds, sterilize with 75Vol% alcohol for 20-40s, wash with sterile water 1-3 times , 0.1wt% mercuric chloride sterilization treatment for 3 to 8 minutes, washed with sterile water for 5 to 6 times, to obtain explants after sterilization;

[0043] Initiating culture: inoculate the sterilized explants in the initiating medium for initiating culture to obtain elongated axillary buds with a length of 1-1.5 cm; the initiating medium includes: WPM salts and H organic, supplemented with TDZ, NAA, PPM, 20-30g / L sucrose, 4-6g / L agar. The pH value of the start-up medium is 5.5-5.8; the culture temperature of the start-up culture process is 24-26°C, the light intensity is 1500-2000Lx, and the light time is 12-14h / d;

[0044] According to the TDZ concentration, NAA concentrati...

Embodiment 3

[0049] Effects of Growth Hormone Concentration in Proliferation Medium on Proliferation of Axillary Buds

[0050] Pretreatment: Take the branches of Acer palmatum in the same year, remove the leaves, cut into budded stems with 1-2 buds, sterilize with 75Vol% alcohol for 20-40s, wash with sterile water 1-3 times , 0.1wt% mercuric chloride sterilization treatment for 3 to 8 minutes, washed with sterile water for 5 to 6 times, to obtain explants after sterilization;

[0051] Initiating culture: inoculate the sterilized explants in the initiating medium for initiating culture to obtain elongated axillary buds with a length of 1.0-1.5 cm; the initiating medium includes: WPM salts and H organic, supplemented with 0.005~0.01mg / LTDZ, 0.05mg / LNAA, 0.2%PPM, 20~30g / L sucrose, 4~6g / L agar;

[0052] Proliferation culture: inoculate the axillary buds in the proliferation medium for proliferation culture to obtain sterile sprouts with a plant height of 1.0-2.0 cm; the proliferation medium i...

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Abstract

The invention discloses a tissue culture and rapid propagation method for echeveria nodulosa of Japanese maple. The tissue culture and rapid propagation method for echeveria nodulosa of Japanese maplecomprises the following steps: pretreatment: taking an annual shoot of the echeveria nodulosa of the Japanese maple, removing leaves, shearing the annual shoot into a stem with a bud, and carrying out sterilizing and cleaning treatment to obtain a sterilized explant; initiation culture: inoculating the sterilized explant on an initiation culture medium and carrying out initiation culture to obtain stretched-out axillary buds; propagation culture: inoculating the axillary buds on a propagation culture medium and carrying out propagation culture to obtain sterile buds; and rooting culture: cutting off tissue culture from the bases of the cluster buds, then inoculating the cluster buds on a rooting culture medium, and carrying out rooting culture to obtain test-tube plantlets. The tissue culture and rapid propagation method is short in culture time, a culture process is simple and convenient, the breeding efficiency of the echeveria nodulosa of the Japanese maple is improved, the survival rate of the induced axillary buds is 98% or above, the growth coefficient is 5-7, and the rooting rate is 95% or above. The test-tube plantlets which are consistent in inheritable character can be obtained rapidly. Requirements of large-scale industrialized seedling culture and industrialized development can be met.

Description

technical field [0001] The invention relates to the technical field of tissue culture rapid propagation, in particular to a method for tissue culture rapid propagation of Acer palmatum. Background technique [0002] Acer palmatum 'beni-tsukasa' is a cultivar of Acer palmatum (Acerpalmatum), a deciduous shrub with an erect trunk and a dome-shaped crown. It grows in zones 5-9. Mature plant height and crown width 3-3.6m, flowering in April, red flowers, full sun or partial shade, grow well in neutral or slightly acidic fertile sandy loam with good drainage. Palmate leaves, 5-7 lobes, lobes as deep as 1 / 2 from the base of the leaves, with heavily serrate margins. The foliage changes from bright peach to yellow and yellow-red in spring, green with pink and red hues in late spring, green and dark green with variegated foliage in summer when mature, and red and orange in fall. It blooms in spring and the flowers are red. [0003] Due to the characteristics of leaf color changes ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 蔡世林杨金财赵玲张秀秀
Owner SICHUAN COLORLINK CO LTD