Composition containing stem cell component and application thereof

A technology of stem cells and compositions, which is applied in the field of compositions containing stem cells, can solve problems such as the inability to prevent human beings from pursuing beauty, and achieve good effects, anti-oxidation and anti-aging effects

Inactive Publication Date: 2018-05-25
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Aging research has become a major research topic in the field of life sciences. Even though aging is inevitable, it cannot prevent human beings from pursuing the right to beauty

Method used

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  • Composition containing stem cell component and application thereof
  • Composition containing stem cell component and application thereof
  • Composition containing stem cell component and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0019] Preparation of stem cell concentrate: collect the cell culture supernatant before passage to obtain stem cell culture; filter to remove cell debris and microorganisms; place the filtrate in a 5K ultrafiltration tube at low temperature (-4°C to 8°C) and high speed Centrifuge at a speed of 800-5000 r / min to obtain a stem cell concentrate, which is stored at -20°C for future use. The stem cells in this experimental example are embryonic stem cells.

[0020] Activity detection method: H 2 o 2 Induced Cellular Senescence Model

[0021] 1.H 2 o 2 Establishment of induced cell senescence model, established H 2 o 2 Stimulation time and concentration

[0022] Facic acid dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to insoluble blue-purple formazan, and the amount of blue-purple formazan formed is proportional to the number of living cells and the biosynthetic metabolic capacity of cells, while dead cells No such function. Therefore, the sur...

Embodiment 1

[0032] A composition containing stem cell components, based on a total mass fraction of 100%, comprising 20% ​​of stem cell concentrate and 80% of high-sugar DMEM complete medium, and 1 μmol / mL of vitamin E and 0.0001-1 μg / mL of PDGF . In this embodiment, the concentrated solution of stem cells is the concentrated solution of embryonic stem cells. Embryonic stem cells were obtained from the Cell Bank of the Chinese Academy of Sciences.

[0033] Several groups of compositions were designed by using the SAS method, which are:

[0034] (1) Stem cell concentrate with a mass fraction of 20% and high-sugar DMEM complete medium with a mass fraction of 80%, with 1 μmol / mL of vitamin E and 0.0001 μg / mL of PDGF added;

[0035] (2) Stem cell concentrate with a mass fraction of 20% and high-sugar DMEM complete medium with a mass fraction of 80%, with 1 μmol / mL of vitamin E and 0.001 μg / mL of PDGF added;

[0036] (3) Stem cell concentrate with a mass fraction of 20% and high-sugar DMEM ...

Embodiment 2

[0044] A composition containing stem cell components, based on a total mass fraction of 100%, comprising 40% of stem cell concentrate and 60% of high-sugar DMEM complete medium, and vitamin E with a concentration of 0.01-100 μmol / mL and vitamin E with a concentration of 0.01 μg / mL of PDGF. In this embodiment, the stem cell concentrate is the adult stem cell concentrate. Adult stem cells were obtained from the Guangdong Provincial Cord Blood Hematopoietic Stem Cell Bank.

[0045] Several groups of compositions were designed by using the SAS method, which are:

[0046] (1) Stem cell concentrate with a mass fraction of 40% and high-sugar DMEM complete medium with a mass fraction of 60%, with vitamin E at a concentration of 0.01 μmol / mL and PDGF at a concentration of 0.01 μg / mL;

[0047] (2) Stem cell concentrate with a mass fraction of 40% and high-sugar DMEM complete medium with a mass fraction of 60%, with vitamin E at a concentration of 0.1 μmol / mL and PDGF at a concentratio...

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Abstract

The invention discloses a composition containing a stem cell component and application thereof. The composition is prepared from the following components in percentage by mass: 20 to 100 percent of stem cell concentrated solution and 0 to 80 percent of complete culture medium, totaling 100 percent, wherein vitamin E and a PDGF (Platelet Derived Growth Factor) are added into the composition. The antioxidizing performance of a product is measured through an antioxidizing experiment, and an oxidizing stress cell aging model is built to research the anti-aging function of the stem cell composition, thereby finding the stem cell composition which has a plurality of effects, small toxic and side effects and an anti-aging function.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a composition containing stem cell components and its application. Background technique: [0002] Aging research has become a major research topic in the field of life sciences. Even though aging is inevitable, it cannot stop human beings from pursuing the right to beauty. In recent years, the research on anti-aging drugs has been the focus of people's attention. Anti-aging drugs are an important tool for delaying aging and aiming at improving life efficiency. Studies have shown that antioxidants can scavenge free radicals, prevent free radicals from destroying biofilms, and have a certain anti-aging effect. Antioxidant experimental tests are carried out on selected drugs. Studies have shown that stem cells, natural products and many compounds have anti-aging effects, and skin aging is a direct response to the aging of the body. Therefore, human skin fibroblast HSF is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0735C12N5/0789C12N5/10A61K8/99A61Q19/08A61K35/545A61K35/28A61P39/06
Inventor 李志远王飞程娜黄荣奇林佐贤李帅黄华林阳雨辰田超赵惠芳
Owner GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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