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Application of small molecules produced by X. citri as synergists of thiazole fungicides in disease control

A technology of citrus canker and biosynergist, which is applied in the direction of fungicides, applications, biocides, etc., can solve the problems of no research on the bactericidal effect of small molecule compounds, and achieve the effect of reducing the amount of medicine and improving the efficacy

Active Publication Date: 2020-07-31
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But there is no report on improving the bactericidal effect of the medicament on the small molecular compound

Method used

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  • Application of small molecules produced by X. citri as synergists of thiazole fungicides in disease control
  • Application of small molecules produced by X. citri as synergists of thiazole fungicides in disease control
  • Application of small molecules produced by X. citri as synergists of thiazole fungicides in disease control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 4

[0029]The mother solution concentration of 30% zinc thiazole used in Example 4 is 10 mg / mL, and the working concentration is a 500-fold dilution. The small molecule was tested at a concentration of 100 mM. The embodiments of the present invention show that small molecular compounds and thiazole chemical reagents in a certain concentration range are effective against rice base rot pathogens as biological source synergists. EC1 There is a certain synergistic bactericidal effect in the prevention and treatment of bactericide, and the combination of some thiazole fungicides and small molecular compounds in accordance with the technical characteristics of the present invention can achieve this synergistic effect as a synergist.

Embodiment 1

[0030] Embodiment 1 DSF and Xf Preparation of DSF

[0031] cultured overnight Xac The seed solution was inserted into the YEB medium, and after culturing at 28°C and 200rpm for 24 hours, the X. citrus bacteria were collected by centrifugation Xac culture supernatant. The obtained supernatant is used for extraction of small molecules.

[0032] The present invention adopts the conventional compound separation and extraction method to separate and extract the active substances in the fermentation broth (for specific methods, please refer to the two prior art documents mentioned in the background technology of the description). Canker sorrel citri Xac After the supernatant of the culture solution, add the same volume of ethyl acetate as the supernatant, let it stand at 4°C for 2h, then shake it at 20°C for 1h, extract it with a separatory funnel, and collect the upper layer. The collected upper layer was suspended and evaporated with a rotary evaporator, and the organic s...

Embodiment 2

[0033] Example 2 Small molecule compound mixed with 30% thiazole zinc against canker sores citrus Xac bactericidal synergistic effect

[0034] Use methanol as a solvent to dissolve the extract, mix it with 10mg / mL thiazole zinc reagent to treat the citrus canker sore liquid with a certain OD value, and use the total number of bacterial communities (CFU) per unit volume to measure the bactericidal synergistic effect of different concentrations of extracts on thiazole zinc . The specific operation is:

[0035] Statistical CFU method is used: use methanol as a solvent to prepare the small molecule compound into a certain concentration of the test agent, accurately add a certain amount of small molecule compound solution and mix 500 times the thiazole zinc reagent to cultivate overnight to OD 600 =1.0 of Xac In the bacterial solution, treat different concentrations of small molecule compounds, 500 times thiazole zinc reagent and the bacterial solution at the same time, and tr...

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PUM

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Abstract

The invention discloses application of a small molecule compound (DSF) produced by Xanthomonas axonopodis pv. citri (Xac) as a synergist for thiazole bactericides. Specifically, as the small moleculecompound with a certain concentration is added into commercially available mainstream thiazole bactericides, the bactericidal effect of the thiazole bactericides can be substantially improved. DSF isextracted from the fermentation broth of Xanthomonas axonopodis pv. citri (Xac), and extraction and separation are easy, or DSF may be artificially or chemically synthesized. The synergist provided bythe invention can substantially improve the bactericidal effect of thiazoles, greatly reduce the usage amounts of chemical pesticides, weakens the pesticide resistance of pathogens and eventually promote harmonious development of the planting industry and environment.

Description

technical field [0001] The invention relates to the field of plant protection of biological control technology, in particular to canker sores citrus Xanthomonas axonopodis pv. citri ( Xac ) The small molecular compound produced as a green and safe new synergist can improve the bactericidal effect of thiazole fungicides such as thiazole zinc, and reduce the application of chemical pesticides in disease control. Background technique [0002] In the comprehensive prevention and control of plant diseases, chemical pesticide control is widely favored because of its fast timeliness and remarkable effect. China is one of the countries with the most serious abuse of chemical agents in the world. As people pay more and more attention to personal health, food safety and ecological environment, it is imperative to reduce the use of chemical agents in agricultural production, especially the traditional high toxicity and high residue chemical pesticides. Research and development a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/06A01N43/824A01P3/00
CPCA01N37/06A01N43/82
Inventor 常长青邓嘉茹姜子德廖金星陈少华王金佩蔡恩平
Owner SOUTH CHINA AGRI UNIV
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