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A kind of preparation method of edible fungus fermentation product rich in stilbene compounds

A technology of stilbene compounds and fermentation products, which is applied in the field of bioengineering to achieve the effects of wide sources, high biological potency and low cost

Active Publication Date: 2020-09-25
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other uses of cold shock protein have not been reported yet, and some applied research is yet to be carried out

Method used

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  • A kind of preparation method of edible fungus fermentation product rich in stilbene compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1. Material preparation

[0050] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0051] The Phellinus strains preserved on the slant were inoculated on the PDA plate medium and activated for 10 days at 22° C. to obtain the activated Phellinus strains.

[0052] Liquid seed medium: glucose 12g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.

[0053] The solid medium is composed of the following components by mass percentage: yeast powder 0.4%, peptone 0.2%, K 2 HPO 4 0.2%, MgSO 4 0.1% and the rest of water, with natural pH, sterilized at 121°C for 20min.

[0054] The composition of the liquid fermentation medium is: glucose 18g, peptone 6g, beef extract 2g, KH 2 PO 4 1.2g and MgSO 4 0.75g, dilute to 1000mL with water, the pH is natural, and sterilize at 121°C for 20min.

[0055] Pre...

Embodiment 2

[0061] 1. Material preparation

[0062] PDA plate medium: potato 200g, glucose 20g and agar 20g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0063] The Phellinus strains preserved on the slant were inoculated on the PDA plate medium and activated for 3 days at 30° C. to obtain the activated Phellinus strains.

[0064] Liquid seed medium: glucose 5g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.

[0065] The solid medium is composed of the following components in mass percentage: yeast powder 1%, peptone 0.2%, K 2 HPO 4 0.1%, MgSO 4 0.15% and the rest of water, with natural pH, sterilized at 121°C for 20min.

[0066] The composition of the liquid fermentation medium is: glucose 18g, peptone 6g, beef extract 2g, KH 2 PO 4 1.2g and MgSO 4 0.75g, dilute to 1000mL with water, the pH is natural, and sterilize at 121°C for 20min.

[0067] Prepar...

Embodiment 3

[0073] 1. Material preparation

[0074] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0075] The Phellinus strains preserved on the slant were inoculated on the PDA plate medium and activated for 6 days at 26° C. to obtain the activated Phellinus strains.

[0076] Liquid seed medium: glucose 20g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.

[0077] The solid medium is composed of the following components in mass percentage: yeast powder 0.7%, peptone 0.8%, K 2 HPO 4 0.25%, MgSO 4 0.05% and the rest of water, with natural pH, sterilized at 121°C for 20min.

[0078] The composition of the liquid fermentation medium is: glucose 18g, peptone 6g, beef extract 2g, KH 2 PO 4 1.2g and MgSO 4 0.75g, dilute to 1000mL with water, the pH is natural, and sterilize at 121°C for 20min.

[0079] Pr...

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Abstract

The invention discloses a preparation method of an edible and medicinal fungus fermentation product containing rich stilbene compounds. The preparation method comprises the following steps: inoculating phellinus igniarius seed liquid into a solid culture medium added with an exogenous substance and culturing; after drying and crushing a cultured product, obtaining an exogenous substance solid fermentation culture substance, wherein the exogenous substance is selected from one or more than two of peanut, mulberry fruit, grape, radix polygoni multiflori and radix et rhizoma rhei; inoculating thephellinus igniarius seed liquid into a liquid fermentation culture medium added with the exogenous substance solid fermentation culture substance and culturing for a period of time; adding cold shockprotein and then continually culturing for a period of time; then stopping fermentation to obtain the edible and medicinal fungus fermentation product containing the rich stilbene compounds. According to the preparation method disclosed by the invention, the exogenous substance contains less resveratrol and is combined with characteristic of a stilbene compound anabolism way, and phellinus igniarius solid fermentation and liquid fermentation technologies are carried out; the cold shock protein is added at a specific fermentation phase, so that the amount of the biologically synthesized stilbene compounds can be effectively increased and a new way is provided for the product containing the rich stilbene compounds.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for preparing an edible fungus fermentation product rich in stilbene compounds. Background technique [0002] Phellinus igniarius belongs to Basidiomycotina, Phellinus class, Rustiaceae, Pinifera. Phellinus extract has significant effects in the clinical application of inhibiting cancer cell metastasis and preventing cancer recurrence after surgery. It is currently an edible fungus with the highest efficiency among internationally recognized biological cancer treatment agents. [0003] Due to the particularity and complexity of the physiological state and the constraints of the external environment, Phellinus seldom forms fruiting bodies in nature, especially it takes many years to form usable fruiting bodies. Moreover, artificial cultivation is extremely difficult, the cultivation conditions are harsh, and the growth cycle is as long as 3-4 years, which is dif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/22C12R1/645
CPCC12P7/22
Inventor 程俊文贺亮魏海龙胡传久王衍彬李海波邹景泉
Owner ZHEJIANG FORESTRY ACAD
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