Automatic western blot analyzer

Abstract

The invention relates to an automatic immunoblotting analyzer, comprising: a reagent module, a sample injection module, a material addition module, a cleaning module, an incubation module, a liquid injection and suction module, an interpretation module, and a drying module. At the same time, a fully automatic immunoblotting analysis test method is also provided. The beneficial effects of the present invention are as follows: with the incubation module as the core, the other modules are arranged around the incubation module, according to the tested items, the sample injection module, the reagent module and the feeding module are used to realize automatic sample feeding and automatic addition of reagents, and the cleaning module is used to The feeding module is cleaned to avoid contamination between the agents. The incubation module is used to inject the cleaning solution and the waste liquid is removed. The drying module is used to dry the incubation module. Image analysis and result output do not require manual participation in the test process, and each module cooperates with each other to realize the whole process from sample injection to result output.

Description

Technical field [0001] The invention relates to the technical field of detection equipment, in particular to an automatic western blotting analyzer. Background technique [0002] Immunoblotting, also known as Western blotting, is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. It is developed on the basis of gel electrophoresis and solid-phase immunoassay technology. A new immunological biochemical technology. Western blot is often used to identify a certain protein, and can perform qualitative and semi-quantitative analysis of the protein. Combined with chemiluminescence detection, it can compare the expression differences of the same protein in multiple samples at the same time. The immunoblotting method detects the specific immune reaction between the antibody and the antigen epitope presented by the target protein attached to the solid support. The whole process mainly includes: protein separation, electrotr...

AI Technical Summary

Problems solved by technology

[0003] The detection method of the traditional Western blot analyzer is generally manual, and different reagents need to be replaced. Frequent operations increase the work intensity and time of the experimenter, and the manual operation is cumbersome and complicated, and there is a lot of contact between people and potentially infectious objects. poor security

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