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Marine Streptomyces s063 and its anti-complement activity application

A marine Streptomyces anti-complement technology, applied in the field of microorganisms, can solve the problems of unknown targets, toxic and side effects, and unclear structure of anti-complement active ingredients, and achieve the effect of protecting blood cells and simple preparation

Active Publication Date: 2021-03-12
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the research on anti-complement active substances derived from natural products is still in its infancy, and there are still many problems to be solved. For example, the structures of anti-complement active ingredients isolated from many natural products are not clear, and the targets and toxic side effects are unknown.

Method used

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  • Marine Streptomyces s063 and its anti-complement activity application
  • Marine Streptomyces s063 and its anti-complement activity application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, identification of bacterial strain S063

[0038] Based on the morphological characteristics, physiological and biochemical characteristics, and 16S rRNA sequence of Streptomyces, it was identified as Streptomyces. The specific identification results are as follows:

[0039] 1. Cell morphology

[0040] Strain S063 grew well on Bennett's medium with raised, wrinkled colonies and white spores (see figure 1 ).

[0041] 2. Physiological and biochemical characteristics

[0042] Streptomyces S063 can grow on the soybean tryptone medium with an additional salt concentration of 0-12%. It grows well and produces spores at a salt concentration of 0-9%. When it reaches 12%, the colony is very small and grows slowly.

[0043] Table 1 Salt tolerance of actinomycetes S063

[0044] NaCl content (%) 0 3 6 9 12 Growth Status ++ +++ ++ ++ +

[0045] Note: The more "+" number, the better the growth

[0046] 3.16S rRNA Identification

[0...

Embodiment 2

[0050] Example 2, preparation of metabolites of marine Streptomyces S063 with anti-complement effect

[0051] (1) Fermentation of Streptomyces S063

[0052] C, the preparation of marine Streptomyces S063 seed solution: insert the mycelia and spores on the plate into soybean tryptone medium, cultivate for 48 hours at 30° C. at 200 rpm to obtain the seed solution;

[0053] D. Fermentation of marine Streptomyces S063: insert 0.1 mL inoculum of the seed liquid into 15 mL of the solid medium agar plate, including No. 2, No. 3, No. 8, No. 17 and No. 19 media , cultured at 30°C for 7 days.

[0054] E. Liquid fermentation culture of Streptomyces marinum S063: Inoculate 1 mL of the seed liquid into 50 mL soybean tryptone fermentation medium, and culture at 30° C. for 7 days.

[0055] The composition of the soybean tryptone medium (1#): tryptone 17g, soybean peptone 3g, sodium chloride 5g, glucose 2.5g, dipotassium hydrogen phosphate 2.5g, distilled water to 1L, 121°C damp heat ster...

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Abstract

The invention discloses a strain of marine streptomyces S063 and its anti-complement activity application; the marine streptomyces is marine streptomyces (Streptomyces sp.) S063 CGMCC No.14582. For the marine Streptomyces of the present invention, the complement hemolysis test shows that its fermentation liquid has significant anti-complement activity. The invention also discloses the fermentation conditions for preparing the anti-complement active substance of the marine streptomyces.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of marine streptomyces S063 and its anti-complement activity application. Background technique [0002] The marine environment occupies 70.8% of the total area of ​​the earth and contains rich microbial resources. The unique environmental characteristics of the ocean, such as low temperature, low nutrition, high pressure, and high salinity, have created the unique metabolic methods and metabolites of marine microorganisms. Bacteria have the ability to synthesize a variety of secondary metabolites due to their complex morphological differentiation process. In recent years, with the development of molecular biology techniques, more and more marine actinomycetes have been discovered, and many secondary metabolites derived from marine actinomycetes have also been identified to have antitumor, antibacterial, antimalarial and killing properties. insect function, and various ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/741A61P7/00C12R1/465
CPCA61K2035/115C12N1/205C12R2001/465
Inventor 赵心清
Owner SHANGHAI JIAO TONG UNIV
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