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Marine Streptomyces dut11 and its anti-complement activity application

A marine Streptomyces, DUT11 technology, applied in the field of microorganisms, can solve the problems of unclear structure of anti-complement active ingredients, unknown targets of action, and unknown toxic and side effects, and achieve the effect of protecting blood cells and simple preparation

Active Publication Date: 2020-12-08
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the research on anti-complement active substances derived from natural products is still in its infancy, and there are still many problems to be solved. For example, the structures of anti-complement active ingredients isolated from many natural products are not clear, and the targets and toxic side effects are unknown.

Method used

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  • Marine Streptomyces dut11 and its anti-complement activity application
  • Marine Streptomyces dut11 and its anti-complement activity application
  • Marine Streptomyces dut11 and its anti-complement activity application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, identification of bacterial strain DUT11

[0043] Based on the morphological characteristics, physiological and biochemical characteristics, and 16S rRNA sequence of Streptomyces, the strain was identified as Streptomyces. The specific identification results are as follows:

[0044] 1. Cell morphology

[0045] Strain DUT11 grew well on Bennett's medium with raised, wrinkled colonies and white spores (see figure 1 ), while DUT11 produces a yellow pigment.

[0046] 2. Physiological and biochemical characteristics

[0047] Streptomyces DUT11 can grow on soybean tryptone medium with an additional salt concentration of 0-15%. It grows well and produces spores at a salt concentration of 0-8%. When it reaches 15%, the colonies are very small and grow slowly, basically not grow.

[0048] Table 1 Salt tolerance of actinomycetes DUT11

[0049] NaCl content (%) 0 3 5 8 10 15 Growth Status ++ ++ +++ ++ + +

[0050] Note: The mor...

Embodiment 2

[0056] Example 2, Preparation of Metabolites of Marine Streptomyces DUT11 with Anti-complement Effect

[0057] (1) Fermentation of Streptomyces DUT11

[0058] A. Preparation of Streptomyces DUT11 seed solution: insert the mycelia and spores on the plate into soybean tryptone medium, cultivate at 30°C and 200rpm for 48h to obtain the seed solution;

[0059] B. Fermentation of Streptomyces DUT11: Inoculate 1 mL of Streptomyces DUT11 seed solution into 50 mL of fermentation medium, including soybean tryptone, No. 3, No. 4, No. 5, No. 9 and No. 33 media, 30 Cultivate at ℃ for 7 days;

[0060] The composition of the soybean tryptone medium (1#): tryptone 17g, soybean peptone 3g, sodium chloride 5g, glucose 2.5g, dipotassium hydrogen phosphate 2.5g, distilled water to 1L, 121°C damp heat sterilization for 20min;

[0061] The No. 3 medium (3#) consists of: 20g soluble starch, 25g soybean powder, 2g ammonium sulfate, 2g sodium chloride, 0.5g dipotassium hydrogen phosphate, 5.0g ca...

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Abstract

The invention discloses a strain of marine streptomyces DUT11 and its anti-complement activity application; the marine streptomyces is marine streptomyces (Streptomyces sp.) DUT11 CGMCC No.14581. For the marine Streptomyces of the present invention, the complement hemolysis test shows that its fermentation liquid has significant anti-complement activity. The invention also discloses the fermentation condition for the preparation of the marine streptomyces anti-complement active substance and its metabolites tunicamycin I, V and VII all have anti-complement activity.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of marine streptomyces DUT11 and its anti-complement activity application. Background technique [0002] The marine environment occupies 70.8% of the total area of ​​the earth and contains rich microbial resources. The unique environmental characteristics of the ocean, such as low temperature, low nutrition, high pressure, and high salinity, have created the unique metabolic methods and metabolites of marine microorganisms. Bacteria have the ability to synthesize a variety of secondary metabolites due to their complex morphological differentiation process. In recent years, with the development of molecular biology techniques, more and more marine actinomycetes have been discovered, and many secondary metabolites derived from marine actinomycetes have also been identified to have antitumor, antibacterial, antimalarial, and killing properties. Various biologically active...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/741A61P7/00C12R1/465
CPCA61K2035/11C12N1/205C12R2001/465
Inventor 赵心清
Owner SHANGHAI JIAOTONG UNIV
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