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Platinum-gold nanometer spherical shell-coated triptolide lipidosome and preparation method thereof

A technology of triptolide and gold nanosphere shells, applied in the field of biomedicine, can solve the problems of limiting the wide application of multi-drug combination therapy and release on demand, avoiding multi-drug resistance phenomenon, reducing toxic and side effects, and achieving good light Responsive Effects

Active Publication Date: 2018-06-22
秦皇岛满药本草生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although multi-drug combination therapy has great application prospects, it is difficult to accurately release multiple drugs on specific tumor targets on demand, which limits the wide application of multi-drug combination therapy.

Method used

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  • Platinum-gold nanometer spherical shell-coated triptolide lipidosome and preparation method thereof
  • Platinum-gold nanometer spherical shell-coated triptolide lipidosome and preparation method thereof
  • Platinum-gold nanometer spherical shell-coated triptolide lipidosome and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Dissolve 2mg triptolide (purchased from Chengdu Pufeide Biotechnology Co., Ltd.), 4mg cholesterol (purchased from Tianjin Damao Yitong Supply Station) and 40mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.) In 5mL of absolute ethanol, stir magnetically to obtain a uniform lipid solution; dissolve 4mg of polyethylene glycol in 10mL of pH6 phosphate buffer to obtain a hydration medium; under magnetic stirring, slowly drop 5mL of the above lipid solution into 7.5 mL of hydration medium at 35° C. to obtain triptolide liposome suspension. Then it was placed on a shaker, incubated at 18° C. and 60 r / min for 1 h, and the absolute ethanol was evaporated to obtain polyethylene glycol-modified triptolide liposomes.

[0030] Dissolve 5 mg of glutathione in 1 mL of deionized water to obtain a glutathione solution, and slowly drop 1 mL of glutathione solution into 9 mL of polyethylene glycol-modified liposome solution under magnetic stirring , conti...

Embodiment 2

[0036]Dissolve 2mg triptolide (purchased from Chengdu Pufeide Biotechnology Co., Ltd.), 6mg cholesterol (purchased from Tianjin Damao Yitong Supply Station) and 50mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.) In 5mL of absolute ethanol, stir magnetically to obtain a uniform lipid solution; dissolve 5mg of polyethylene glycol in 10mL of pH6.5 phosphate buffer to obtain a hydration medium; Slowly drop into 10 mL of hydration medium at 40°C to obtain triptolide liposome suspension. Then place it on a shaker, incubate for 2 hours at 20° C., 70 r / min, and evaporate absolute ethanol to obtain polyethylene glycol-modified triptolide liposomes.

[0037] 7.5 mg of glutathione was dissolved in 1 mL of deionized water to obtain a glutathione solution. Under magnetic stirring, 1 mL of the glutathione solution was slowly dropped into 10 mL of the polyethylene glycol-modified liposome solution at a constant speed, and the stirring was continued for 4 ho...

Embodiment 3

[0043] Dissolve 2mg triptolide (purchased from Chengdu Pufeide Biotechnology Co., Ltd.), 8mg cholesterol (purchased from Tianjin Damao Yitong Supply Station) and 60mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.) In 5mL absolute ethanol, stir magnetically to obtain a uniform lipid solution; dissolve 6mg polyethylene glycol in 10mL pH6 phosphate buffer to obtain a hydration medium; under magnetic stirring, slowly drop 5mL of the above lipid solution into 12.5mL of 45°C hydration medium to obtain triptolide liposome suspension. Then place it on a shaker, incubate at 23° C., 80 r / min for 3 h, and evaporate absolute ethanol to obtain polyethylene glycol-modified triptolide liposomes.

[0044] Dissolve 10 mg of glutathione in 1 mL of deionized water to obtain a glutathione solution. Under magnetic stirring, 1 mL of the glutathione solution was slowly dropped into 11 mL of the polyethylene glycol-modified liposome solution at a constant speed, and ...

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Abstract

The invention relates to a platinum-gold nanometer spherical shell-coated triptolide lipidosome. The growth control is performed on the surface of the triptolide lipidosome to from a gold nanometer flower structure; then, a layer of nanometer medicine-carrying lipidosome of a platinum spherical shell structure further grows on the surface in a regulation and control way. The preparation method mainly comprises the following steps that the triptolide lipidosome modified by glutathione is mixed and hatched with gold nanometer particles at a certain proportion; control is performed to obtain different-combination quantity gold nanometer particle-modified triptolide lipidosome; then, the triptolide lipidosome coated by the gold nanometer flowers is obtained through secondary crystal growth; acertain proportion of chloroplatinic acid and reducing agents are added for hatching; the platinum-gold nanometer spherical shell-coated triptolide lipidosome is finally obtained. The prepared platinum-gold nanometer spherical shell-coated triptolide lipidosome has the advantages that the toxic and side effects of the medicine on normal tissues can be effectively reduced; the occurrence of multi-medicine resistance phenomenon of the tumor can be avoided; the obvious tumor cell killing capability is realized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a triptolide liposome and a preparation method thereof. Background technique [0002] In recent years, the morbidity and mortality of cancer have been increasing, and drug resistance in chemotherapy is currently the primary reason that affects the complete treatment of tumors and leads to tumor recurrence. In order to increase the effective concentration of the drug at the tumor site, repeated administration will induce drug resistance of tumor cells and increase the toxic and side effects of the drug on normal tissues. Multi-drug combination therapy can solve the problem of tumor drug resistance and prevent cancer recurrence. By selecting drugs with different mechanisms of action, synergistic anti-tumor effects can be exerted, and the goals of high curative effect, less drug resistance, and mild side effects can be achieved with the lowest effective dose. For example, Gnat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/10A61K33/24A61K31/585A61K41/00A61P35/00
Inventor 高大威骆丽垚刘艳平张旭武李磊何雨初
Owner 秦皇岛满药本草生物科技有限公司
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