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Application of long-chain non-coding RNA LINC00336 as biomarker in preparing lung squamous cell carcinoma prognosis detection preparation

A long-chain non-coding, biomarker technology, applied in the application field of preparation of lung squamous cell carcinoma prognostic detection reagents, can solve the problem of not being able to adapt to the prognosis determination of lung squamous cell carcinoma patients, and having no reference standard or specificity for the prognosis determination of lung squamous cell carcinoma patients. Sexual indicators and other issues, to achieve far-reaching clinical significance and promotion effect

Active Publication Date: 2018-06-22
XIANGYA HOSPITAL CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no reference standard for the prognosis of patients with squamous cell carcinoma of the lung, and there is no specific index, which is far from meeting the needs of the prognosis of patients with squamous cell carcinoma of the lung.

Method used

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  • Application of long-chain non-coding RNA LINC00336 as biomarker in preparing lung squamous cell carcinoma prognosis detection preparation
  • Application of long-chain non-coding RNA LINC00336 as biomarker in preparing lung squamous cell carcinoma prognosis detection preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0013] Example 1 Preparation of long-chain non-coding RNA LINC00336 for lung squamous cell carcinoma prognosis detection kit (50 reactions)

[0014] 1. RNA stabilization solution 50ml

[0015] 2. Isopropanol 100ml

[0016] 3. Chloroform 100ml

[0017] 4. Trizol 50ml

[0018] 5. Enzyme-free water 10ml

[0019] 6.1μM random reverse transcription primer 50ul

[0020] 7.5× reverse transcription buffer 200ml

[0021] 8.10mM base triphosphate deoxynucleotide 100ul

[0022] 9.40U / μl RNase inhibitor 500ul

[0023] 10.200U / μl MMLV reverse transcriptase 50ul

[0024] 11. Premix Ex Taq 50ul

[0025] 12. 10μM long non-coding RNA LINC00336 real-time fluorescent quantitative PCR specific primers:

[0026] Forward primer 5'-CCACTGAGCAGAGGTGCTGGC-3' (SEQ ID NO.2),

[0027] Reverse primer 5'-GTGCAGTGGCTCACGCCTATAA-3' (SEQ ID NO.3),

[0028] 13. 10μM internal reference ACTB real-time fluorescent quantitative PCR specific primers:

[0029] The forward primer is 5'-CACCATTGGCAATGAGCGG...

Embodiment 2

[0031] Example 2 Detection of long non-coding RNA LINC00336 in lung squamous cell carcinoma tissue

[0032] 1. Preservation of lung squamous cell carcinoma tissue: collect the lung squamous cell carcinoma tissue to be tested and store it in a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0033] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After the liquid is in the centrifuge tube, add 200μl / ml chloroform to the centrifuge tube, shake it by hand for 15-30s, place it on ice for 5min, and centrifuge at 12000g at 4°C for 15min; carefully take the upper aqueous phase into a new centrifuge tube, add Mix with 0.5ml of pre-cooled isopropanol, put it in a refrigerator at -20°C for 20min, centrifuge at 12000g at 4°C for 10min...

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Abstract

The invention discloses application of long-chain non-coding RNA LINC00336 as a biomarker in preparing a lung squamous cell carcinoma prognosis detection preparation. The expression level of long-chain non-coding RNA LINC00336 in a lung squamous cell carcinoma patient can be detected, a powerful molecular biology basis is provided for prognostic prediction to the lung squamous cell carcinoma patient, and long-chain non-coding RNA LINC00336 has profound clinical significance and good popularization performance.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and specifically relates to the application of long-chain non-coding RNA LINC00336 as a biomarker in the preparation of prognostic reagents for lung squamous cell carcinoma. Background technique [0002] Lung cancer is one of the malignant tumors with the highest morbidity and mortality in the world. Squamous cell carcinoma of the lung, or squamous cell carcinoma, is the most common type of lung cancer. It is a malignant epithelial tumor derived from the bronchial epithelium. For 40% to 51% of recurrent lung cancer, the 5-year survival rate is still lower than 15% after comprehensive treatment such as surgery, radiotherapy and chemotherapy. At present, there is no reference standard for the prognosis of patients with squamous cell carcinoma of the lung, and there is no specific index, which is far from meeting the needs of determining the prognosis of patients with squamous cell carcinoma o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 陶永光王敏毛超刘双
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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