Column chromatography isolation method for VA acetate, perhydride VA acetate and isomers thereof

Abstract

The invention relates to a column chromatography isolation method for VA acetate, perhydride VA acetate and isomers thereof. The method comprises the following steps: (1) dissolving a VA acetate mother solution into a non-polar solvent in the volume ratio of (1 to 2):1 to obtain a pretreated mother solution; (2) adding the pretreated mother solution in the step (1) onto a silica gel chromatographycolumn, using an eluent to carry out elution, controlling the flow rate to be 5 to 10 mol / min, carrying out qualitative analysis on the eluant by using a liquid chromatogram and ultraviolet spectrophotometer, and collecting high-content VA elutant, wherein the elutant is a mixed liquor of at least one non-polar solvent and at least one polar solvent; (3) carrying out concentration recycling on the VA elutant obtained in the step (2) so as to obtain a high-content refined VA acetate mother solution. The content of the high-content refined VA acetate mother solution obtained by the method can reach 80% or higher, and the yield can reach 90% or higher.

Description

technical field

[0001] The invention relates to a separation process of vitamin A acetate, in particular to a method for separating VA acetate, perhydroVA acetate and its isomers by column chromatography. Background technique

[0002] Vitamin A Acetate (Vitamin A Acetate), also known as vitamin A acetate or retinol acetate, is a kind of light yellow fine spherical particle powder with good fluidity, no peculiar smell, easy to oxidize in the air, and exposed to light Perishable.

[0003] VA acetate is used for vitamin A deficiency. It is an essential factor to regulate the growth and health of epithelial tissue cells. It can maintain the normal metabolism of the human body, maintain the stability and development of cell membranes, and maintain the normal function of the reproductive system. It can be used for skin care, wrinkle removal, Whitening and other advanced cosmetics. At the same time, vitamin A acetate can increase the health of vision, ensure the good comfort of t...