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Sample embedding method

A sample and embedding cassette technology, applied in the biological field, can solve the problems of fragile and incomplete embedded samples, and achieve the effect of simple and easy method and low technical cost.

Inactive Publication Date: 2018-06-29
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of fragile and incomplete embedding samples in the prior art and provide a new sample embedding method, which makes the samples easy to slice and the slices are continuous and complete

Method used

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Embodiment 1

[0032] An embodiment of the sample embedding method of the present invention, the sample embedding method described in this embodiment is:

[0033] (1) Place the 1cm×2cm embedding cassette in a 4°C refrigerator for 10 minutes to completely cool the embedding cassette, that is, the temperature of the cooled embedding cassette is 4°C; wherein, the embedding cassette is wax paper embedding box;

[0034] (2) Add the deep-sea fish gelatin into double-distilled water, and slowly heat until the deep-sea fish gelatin is completely dissolved to obtain a deep-sea fish gelatin aqueous solution, and place the deep-sea fish gelatin aqueous solution in a water bath at 50°C to keep the temperature at 50°C; In the deep-sea fish gelatin aqueous solution, the mass percent of the deep-sea fish gelatin is 10%;

[0035] (3) Implant the aqueous deep-sea fish gelatin solution prepared in step (2) at a temperature of 50°C into the cooled embedding cassette in step (1) from the inlet of the embedding c...

Embodiment 2

[0040] An embodiment of the sample embedding method of the present invention, the sample embedding method described in this embodiment is:

[0041] (1) Place a 1cm×2cm embedding cassette in a 4°C refrigerator for 10 minutes to completely cool the embedding cassette, that is, the temperature of the cooled embedding cassette is 4°C; the embedding cassette is polyethylene embedding box;

[0042] (2) Add the deep-sea fish gelatin into double-distilled water, and slowly heat until the deep-sea fish gelatin is completely dissolved to obtain a deep-sea fish gelatin aqueous solution, and place the deep-sea fish gelatin aqueous solution in a water bath at 50°C to keep the temperature at 50°C; In the deep-sea fish gelatin aqueous solution, the mass percent of the deep-sea fish gelatin is 10%;

[0043] (3) Implant the aqueous deep-sea fish gelatin solution prepared in step (2) at a temperature of 50°C into the cooled embedding cassette in step (1) from the inlet of the embedding cassett...

Embodiment 3

[0048] An embodiment of the sample embedding method of the present invention, the sample embedding method described in this embodiment is:

[0049] (1) Place the embedding cassette in a 0°C refrigerator for 15 minutes to completely cool the embedding cassette, that is, the temperature of the cooled embedding cassette is 0°C; the embedding cassette is a polyethylene embedding cassette;

[0050] (2) Add pigskin gelatin into double-distilled water and heat slowly until the pigskin gelatin is completely dissolved to obtain an aqueous solution of pigskin gelatin. Place the aqueous solution of pigskin gelatin in a water bath at 45° C. to keep its temperature at 45° C.; In the pigskin gelatin aqueous solution, the mass percent of pigskin gelatin is 8%;

[0051] (3) Implant the pigskin gelatin solution prepared in step (2) at a temperature of 45° C. into the cooled embedding cassette in step (1) from the inlet of the cassette to fill half of the space in the groove of the cassette;

...

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Abstract

The invention relates to a sample embedding method and belongs to the technical field of biology. The sample embedding method provided by the invention comprises the following steps: (1) injecting a gelatin solution into a cooled embedding box to fill one part of space of a groove body of the embedding box, wherein each 100mL of the gelatin solution contains 8 to 15g of gelatin; (2) putting a sample to be embedded into the embedding box and then continually injecting a gelatin solution which is identical to the gelatin solution in step (1) into the embedding box until the groove body of the embedding box is fully filled; (3) sealing the embedding box and curing the gelatin solution in the embedding box, so as to obtain a solid embedding object which embeds the sample. After the sample embedding method provided by the invention is used for embedding the sample, a frozen section of the sample is easy to slice, is continuous and complete and has no holes; the sample embedding method has relatively great actual application value. The method provided by the invention is simple and feasible and can be used for highly efficiently obtaining the high-quality frozen section, is applied to imaging analysis of metabolomics and has low technical cost.

Description

technical field [0001] The invention relates to a sample embedding method, which belongs to the field of biotechnology. Background technique [0002] Metabolomics is an emerging systematic discipline after genomics, transcriptomics and proteomics, which mainly studies all small-molecular-weight metabolites of substrates or products of various metabolic pathways after organisms are stimulated or disturbed by the outside world. The study of the changing situation in time and space, and looking for the relative relationship between metabolites and physiological and pathological changes, is an integral part of systems biology. The isolation and identification of important metabolites that can be used as biomarkers is of great significance for understanding biological metabolic pathways. [0003] Biomarkers generally refer to signals that can be used to objectively measure and evaluate abnormal changes in organisms at the molecular, cellular, individual or population levels due ...

Claims

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Application Information

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IPC IPC(8): G01N1/36
CPCG01N1/36G01N2001/364
Inventor 王小兰
Owner GUANGZHOU UNIVERSITY
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