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Compound preparation, feed, or additive and method for degrading aspergillus flavus toxins

A compound preparation and additive technology, which is applied in the field of microorganisms to achieve the effect of simple treatment and content reduction

Active Publication Date: 2018-07-06
COFCO NUTRITION & HEALTH RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the removal effect of the above methods on aflatoxin needs to be improved, so it is urgent to develop a preparation and method that can save energy, protect the environment and efficiently degrade aflatoxin

Method used

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  • Compound preparation, feed, or additive and method for degrading aspergillus flavus toxins

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preparation example Construction

[0020] According to the compound preparation of the present invention, the commercially available salt-tolerant Candida spp. and Saccharomyces ruxii can be conventionally cultured separately, then made into dry powder, and then mixed with the dry powder. Specifically, the preparation method of the dry powder mixture may specifically include: cultivating salt-tolerant Candida and Saccharomyces rouxii in liquid YEB medium for 8-15 hours at 32-37°C and 150-200rmp, respectively, so that the resistant The concentration of Candida salina was 1-5×10 9 CFU / ml, the concentration of Saccharomyces luteus is 1-5×10 9 CFU / ml, and then centrifuged to obtain supernatant and precipitate, freeze-dry the precipitate respectively to obtain dry cell powder, and then mix the obtained dry cell powder to obtain a dry powder mixture. Wherein, centrifugation and freeze-drying are conventional methods in the art, and will not be repeated here.

[0021] Salt-tolerant Candida (Candida versatilis) of th...

preparation example

[0049] Commercially available salt-tolerant Candida (purchased from CGMCC, preservation number is: CGMCC No.3790, as described in the patent application CN102197861A) and Saccharomyces rouxii (purchased from CGMCC, preservation number is: CGMCC No. 2.371) were respectively inoculated in liquid YEB medium with 1% inoculum amount, cultivated at 150rmp and 37°C, and the time of cultivation was such that the concentration of salt-tolerant Candida was 10 9 CFU / ml, the concentration of Saccharomyces luteus is 10 9 CFU / ml, and then made into dry powder separately, and then mixed.

[0050] Glucose oxidase was purchased from Sigma Company, the brand name is G7141.

[0051] Table 1

[0052]

Embodiment 1-7

[0054] It is used to illustrate the degradation effect of the compound preparation provided by the invention on aflatoxin.

[0055] Carry out the preparation of compound preparation according to the proportioning of each embodiment in table 1, then add 0.5ppm aflatoxin B1 in the bacterial preparation prepared respectively, then add water so that the solid content is 30% by weight, after adding the toxin, add 0.5ppm aflatoxin B1 After reacting for 2 hours under the conditions of L NaCl concentration, 37°C, and pH value of 7, take the processed sample to detect the concentration of aflatoxin in the sample according to the national standard GBT 30955-2014, and calculate the toxin removal according to the following formula (1) Rate.

[0056] Toxin removal rate=(initial concentration of toxin in the sample-concentration of toxin in the sample after treatment) / initial concentration of toxin in the sample×100% Formula (1).

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Abstract

The invention relates to the field of aspergillus flavus, in particular to a compound preparation, a feed, or an additive and a method for degrading aspergillus flavus toxins. The compound preparationcomprises microzymes and enzymes, wherein the microzymes are candida versatilis and / or zygosaccharomyces rouxii; the enzymes are glucose oxidase. Special kinds of the microzymes and the enzymes are combined for use, so that the aspergillus flavus toxins can be effectively degraded, and particularly can degrade the aspergillus flavus toxins B1 high in carcinogenicity. The invention creatively compounds the microzymes and the enzymes for preparing the compound preparation, the compound preparation can simply treat or can be added to the feed materials, so that the content of aspergillus flavuscan be reduced, and health of bred animals can be facilitated.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a compound preparation, a feed or an additive and a method for degrading aflatoxin. Background technique [0002] Mycotoxins are a class of secondary metabolites produced by toxin-producing fungi during the damage process, mainly including aflatoxin, deoxynivalenol (also known as vomitoxin, DON), zearalenone (ZEN) and Fumonisins (FUM), ochratoxins and T-2 toxins, etc. [0003] In recent years, due to the frequent occurrence of extreme climates in my country, the head blight that mainly harms wheat and corn has become a pandemic, and the pollution of fumonisins (FUM), ochratoxin and T-2 produced by fungi has increased. Excessive wheat and corn are on the rise. Research on the safe use of grains with excessive toxins can not only protect the interests of farmers in our country, but also prevent them from flowing into the grain market and maintain the quality and safety of national gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/189
Inventor 苏会波林海龙熊强李凡陈博朱镜羲唐堂黄锦张子剑
Owner COFCO NUTRITION & HEALTH RES INST