Application of cotton GhPHOT1-1 gene in efficient utilization of light energy

A light energy and gene technology, applied in the fields of application, genetic engineering, botanical equipment and methods, etc., can solve the problems of low utilization rate of light energy and lack of detailed reports.

Active Publication Date: 2018-07-06
HENAN UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Cotton is a light-loving and warm-loving plant. However, the light energy utilization efficiency of the main cotton varieties in most areas of my country is generally low, and there is potential for improvement in many aspects of improving its photosynthetic efficiency.
[0005] In the prior art, there have been preliminary studies on the cloning of genes related to light absorption and utiliz

Method used

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  • Application of cotton GhPHOT1-1 gene in efficient utilization of light energy
  • Application of cotton GhPHOT1-1 gene in efficient utilization of light energy
  • Application of cotton GhPHOT1-1 gene in efficient utilization of light energy

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Embodiment 1

[0032] On the basis of existing research, in order to further confirm and identify cotton GhPHOT1-1 The inventors conducted relevant experiments on the function of genes in improving the light energy utilization efficiency of cotton. The specific experimental verification process is briefly introduced as follows.

[0033] (1) Determination of cotton light energy utilization efficiency index

[0034] (1.1) Photosynthetic rate

[0035] Photosynthetic rate (photosynthetic rate) refers to the speed of photosynthesis to fix carbon dioxide (or generate oxygen), and is an important indicator of photosynthetic efficiency under light-saturated conditions. The level of photosynthetic ability of plants is generally measured by the photosynthetic rate. Under other conditions being the same, a high photosynthetic rate can promote high yield and high light energy utilization efficiency.

[0036]The present invention utilizes the GFS-3000 photosynthetic-fluorescence measurement system to ...

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Abstract

The invention belongs to the technical field of cotton genetic engineering application, and particularly relates to a patent application for new application of a cotton GhPHOT1-1 gene in efficient utilization of plant light energy. The GhPHOT1-1 gene is associated with plant growth and development; after overexpression of the GhPHOT1-1 gene, the maximum light energy conversion efficiency Fv/Fm ofPSII reaction center of transgenic plant leaf blades is significantly increased, and the maximum photochemical efficiency Fv'/Fm' of PSII under light adaptation conditions is improved, that is: the overexpression of the GhPHOT1-1 can improve the efficiency of light energy utilization of transgenic plants. The cotton GhPHOT1-1 gene has a direct correlation with the photosynthetic efficiency enhancement based on a conserved blue receptor response function. Based on the discovery, the cotton's ability to capture light is better enhanced, so that the cotton can maximize the use of light energy, then a molecular breeding method is used for increasing the yield, showing a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of cotton genetic engineering application, in particular to cotton GhPHOT1-1 A patent application for the new application of genes in the efficient use of light energy in plants. Background technique [0002] Cotton is Malvaceae ( Malvaceae ) Cotton ( Gossypium ) is a plant that can provide renewable resources such as natural fiber, oil and protein for people's daily life, and is one of the most important economic crops in the world. [0003] With the increase of my country's population, the demand for cotton is also increasing day by day, while the area of ​​arable land in our country is decreasing day by day. How to increase cotton production on the limited arable land is a major issue related to the national economy and people's livelihood. Through traditional genetic breeding methods, breeders have bred cotton varieties with improved yield and quality, but it is difficult to further increase the yie...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00A01H6/46
CPCC12N15/8269
Inventor 张骁赵翔高歌尚保栓陶传武郭西宁张飞
Owner HENAN UNIVERSITY
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