Novel colorimetric sensing method for dual pathogenic bacteria

Abstract

The invention discloses a novel colorimetric sensing method for dual pathogenic bacteria, and the novel colorimetric sensing method is used for supersensitive detection of salmonella and staphylococcus aureus. The method establishes an ultra-quick PCR reaction system, so that a conventional PCR process with consumed time of about three hours is reduced to 5 minutes, and therefore, consumed time ofPCR reaction is remarkably reduced; the ultra-quick PCR reaction system loads a nucleic acid self-assembly developing module, so that a reaction signal is amplified again, supersensitive detection ofpathogenic bacteria is favorably realized, and the problem that the conventional PCR results are difficult for visual detection is solved. Compared with a conventional method, the detecting method and a biosensor are quicker, are more sensitive, are stronger in specificity, and are high in sensitivity, and detected results are reliable, so that analyzing and detecting steps can be simplified, analysis time is shortened, and above all, online real-time detection is possible, therefore, carrying and field operating are convenient in the microbiological detection field of the food safety and quick detecting field, and the application prospect is very good.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a new method for colorimetric sensing of dual pathogenic bacteria. Background technique [0002] The traditional bacterial detection method is mainly based on the physiological and biochemical characteristics of the bacteria. After the steps of pre-enrichment, selective plate separation, and biochemical identification, it takes 5-7 days from sampling to confirming the results. The detection cycle is long, the operation is cumbersome, and the workload is heavy. . Using the specificity of antigen-antibody reaction to identify bacteria has a history of more than half a century, but the screening of microbial antibodies is very cumbersome, and the final detection specificity is not high. The continuous improvement and development of molecular biology detection technology has overcome the problems of cumbersome and time-consuming experimental operations of tr...

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Problems solved by technology

[0002] The traditional bacterial detection method is mainly based on the physiological and biochemical characteristics of the bacteria. After the steps of pre-enrichment, selective plate separation, and biochemical identification, it takes 5-7 days from sampling to confirming the results. The detection cycle is long, the operation is cumbersome, and the workload is heavy.

Using the specificity of antigen-antibody reaction to identify bacteria has a history of more than half a century, but the screenin

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