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Seed tissue culture propagation method of polygonatum sibiricum

A technology of seed group and sealwort, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of easy infection of viruses, difficult collection of seeds, low reproduction rate, etc., shorten the germination time, the method is simple and easy, Overcoming the effect of low germination rate

Inactive Publication Date: 2018-07-10
ALPHA FUJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Due to factors such as long breeding time of Polygonatum seeds, low reproduction rate, and difficult collection of seeds, the cost of artificial large-scale planting has greatly increased
Therefore, in traditional artificial planting, asexual reproduction is mainly used, and rhizome propagation is used. After multi-generation reproduction, the degradation is serious and it is susceptible to viruses; at the same time, the amount is large, and wild Polygonatum multiflora needs to be excavated in large quantities, which seriously damages the source of provenance and the environment.
The use of seeds for propagation has a long growth cycle (4 to 5 years from sowing to harvesting), low seed germination rate, and low seedling rate.
Therefore, the conventional propagation method has not adapted to the needs of the rapid development of Polygonatum multiflora industry, and there is an urgent need to develop a method for raising a large number of seedlings quickly and in a short period of time to improve the germination rate.

Method used

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  • Seed tissue culture propagation method of polygonatum sibiricum
  • Seed tissue culture propagation method of polygonatum sibiricum
  • Seed tissue culture propagation method of polygonatum sibiricum

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Experimental program
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Embodiment 1

[0031] The present embodiment provides a seed tissue culture breeding method of Polygonatum multiflora, which specifically includes the following steps:

[0032] 1. Ethylene treatment of Polygonatum multiflora seeds

[0033] Around October, harvest mature Polygonatum multiflora seeds, rub off the peel, and then wash them with clean water. At the same time, prepare a solution containing ethylene at a ratio of 1000uL ethylene per liter of sterile water, and place the seeds in the ethylene solution containing Soak in water for 2d, take out and dry.

[0034] 2. Gibberellin treatment of Polygonatum multiflora seeds

[0035] After mixing equal volumes of 40mg / L gibberellin aqueous solution and 150mg / L carbendazim aqueous solution, soak the Polygonatum polyflora seeds treated in step 1 for 1-3 hours, filter them out, put the seeds in gauze Let it stand for 1 to 3 days at a humidity of 30 to 50%.

[0036] 3. Sterilization of Polygonatum multiflora seeds

[0037] Wash the Polygonat...

Embodiment 2

[0045] The present embodiment provides a seed tissue culture breeding method of Polygonatum multiflora, which specifically includes the following steps:

[0046] 1. Ethylene treatment of Polygonatum multiflora seeds

[0047] Around October, harvest mature Polygonatum multiflora seeds, rub off the peel, and then wash them with clean water. At the same time, prepare a solution containing ethylene at a ratio of 1000uL ethylene per liter of sterile water, and place the seeds in the ethylene solution containing Soak in water for 2d, take out and dry.

[0048] 2. Gibberellin treatment of Polygonatum multiflora seeds

[0049] After mixing equal volumes of 40mg / L gibberellin aqueous solution and 150mg / L carbendazim aqueous solution, soak the Polygonatum polyflora seeds treated in step 1 for 1-3 hours, filter them out, put the seeds in gauze Let it stand for 1 to 3 days at a humidity of 30 to 50%.

[0050] 3. Sterilization of Polygonatum multiflora seeds

[0051]Wash the Polygonatu...

Embodiment 3

[0058] The present embodiment provides a seed tissue culture breeding method of Polygonatum multiflora, which specifically includes the following steps:

[0059] 1. Ethylene treatment of Polygonatum multiflora seeds

[0060] Around October, harvest mature Polygonatum multiflora seeds, rub off the peel, and then wash them with clean water. At the same time, prepare a solution containing ethylene at a ratio of 1000uL ethylene per liter of sterile water, and place the seeds in the ethylene solution containing Soak in water for 2d, take out and dry.

[0061] 2. Gibberellin treatment of Polygonatum multiflora seeds

[0062] After mixing equal volumes of 40mg / L gibberellin aqueous solution and 150mg / L carbendazim aqueous solution, soak the Polygonatum polyflora seeds treated in step 1 for 1-3 hours, filter them out, put the seeds in gauze Let it stand for 1 to 3 days at a humidity of 30 to 50%.

[0063] 3. Sterilization of Polygonatum multiflora seeds

[0064] Wash the Polygonat...

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Abstract

The invention provides a seed tissue culture propagation method of polygonatum sibiricum. The seed tissue culture propagation method comprises the following steps: S1: carrying out ethylene treatment,gibberellin treatment and sterilization treatment on ripe polygonatum sibiricum seeds in sequence; S2: inoculating a tissue culture medium with the polygonatum sibiricum seeds subjected to the sterilization treatment in step S1; culturing under conditions that the temperature is 23 DEG C, the illumination intensity is 1800lux, the illumination time is 14h and the air relative humidity is 40 to 60percent; S3: after the polygonatum sibiricum seeds cultured by step S2 grow to obtain one leaf, carrying out germination management and controlling germination management conditions that the temperature is 25 DEG C, the illumination intensity is 1200lux, the illumination time is 12h and the air relative humidity is 40 to 60 percent. The method provided by the invention is simple and feasible andis low in cost; a seedling culture manner of promoting the seeds to sprout through a hormone action is adopted so that a process of inoculating with polygonatum cyrtonema seeds to obtain seedling plants only needs about 6 month and the sprouting rate can reach 95 percent or more at maximum; the sprouting rate of the polygonatum cyrtonema seeds is improved and the sprouting time of the olygonatum cyrtonema seeds is shortened.

Description

technical field [0001] The invention relates to a seed tissue culture and breeding method of Rhizoma Polygonatum, which belongs to the technical field of breeding of traditional Chinese medicinal materials. Background technique [0002] Polygona-tum sibiricum Red. is a perennial herb of the genus Polygona-tum in the family Liliaceae. It is an understory economic crop integrating edible, medicinal and ornamental values. At present, in our country, Polygonatum Polygonatum, Polygonatum Polyflora, and Polygonatum Polygonatum are used as traditional Chinese medicines. Polygonatum Polygonatum can be divided into three types: Polygonatum Polygonatum, Polygonatum Polygonatum and Rhizoma Rhizoma Rhizoma. Polygonatum Polyflora is the original plant of Polygonatum Polyflora, Polygonatum Polygonatum is the original plant of Polygonatum Polygonatum, and Polygonatum Polygonatum is the original plant of Polygonatum Rhizome. Among them, Polygonatum multiflora has the best quality. Polygon...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 高益槐王忠黄宝菊
Owner ALPHA FUJIAN BIOTECH
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