α-Bisabolol synthetic plasmid and its construction method and Escherichia coli engineering strain

Abstract

The invention discloses an [alpha]-bisabolol synthetic plasmid as well as a construction method and an escherichia coli engineered strain thereof. An IPP and DMAPP synthetic plasmid pS-MVA and the [alpha]-bisabolol synthetic plasmid are constructed, and the two plasmids are introduced into an escherichia coli strain E.coli DH5[alpha]. The [alpha]-bisabolol synthetic plasmid takes a pTrc99A vectoras a skeleton plasmid and is assembled with an escherichia coli gene ispA and an artificially synthetic [alpha]-bisabolol synthetase gene or a wild-type [alpha]-bisabolol synthetase gene from different sources; the artificially synthetic [alpha]-bisabolol synthetase gene is selected from one of AaBBS, LdBBS, MrBBS, ScBBS and SsBBS; and the wild-type [alpha]-bisabolol synthetase gene is selected from one of LdBBS, ScBBS and MrBBS. According to the invention, the [alpha]-bisabolol is synthesized through a biological method; and the [alpha]-bisabolol is high in yield, high in activity and is green-going and environment-friendly.

Description

technical field [0001] The invention belongs to the field of metabolic engineering, and in particular relates to an α-bisabolol synthesis plasmid and its construction method, Escherichia coli engineering strain and application. Background technique [0002] α-bisabolol, also known as bisabolol, is a natural sesquiterpene alcohol that exists in nature. It has several optical isomers as follows: [0003] [0004] They respectively play an important role in industries such as medicine, food and cosmetics. (-)-α-Bisabolol mainly exists in Compositae Chamomile ( Matricaria recutita ) and other plants, have antibacterial, anti-inflammatory, anti-microbial, anti-digestive and other effects. It is used as an active ingredient in skin cosmetics or to suppress skin inflammation to protect and care for allergic skin, etc. [0005] Native American herb sweet tongue ( Lippia dulcis Hernandulcin (hernandulcin) contained in ) is a natural high-efficiency sweetener, and its main ch...

AI Technical Summary

Problems solved by technology

However, as a secondary metabolite, α-bisabolol has a limited natural yield. Obtaining α-bisabolol by direct extraction from biomass is still subject to many factors such as organism growth cycle, environment, and region, and the production The expansion of the scale will endanger the ecological and environmental safety, and even cause the ecological disaster of the disappearance of endangered plants

If chemical synthesis is adopted, the complex chiral chemical structure of α-bisabolol makes direct chemical synthesis difficult, with low purity and poor biological activity

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