Rice black-streaked dwarf disease resistance gene rbsdv-6c and its encoded protein

Abstract

The invention discloses a rice black-streaked dwarf disease resistance gene RBSDV‑6c, its encoded protein and its application. The gene is the CDS sequence shown in SEQ ID NO: 1 in the sequence listing. The encoded protein is the amino acid sequence shown in SEQ ID NO: 3 in the sequence listing. The rice black-streaked dwarf disease resistance gene RBSDV‑6c provided by the invention has important application value, and the gene can be transformed to obtain transgenic rice plants with significantly improved resistance.

Description

Technical field: [0001] The invention belongs to the field of gene sequence and application, in particular to rice black-streaked dwarf resistance gene RBSDV-6c and its encoded protein. Background technique: [0002] Rice is one of the main food crops. Rice black-streaked dwarf disease is an important virus disease of rice caused by rice black-streaked dwarf virus (RBSDV). way to spread. After the rice is infected, it shows typical symptoms such as dwarfing, few tillers, dark green leaves, waxy white to dark brown stripe veins on the back of leaves, leaf sheaths and even stems. Since the 21st century, due to the lack of resistance of the main rice varieties to black-streaked dwarf disease, the disease has become increasingly serious in Jiangsu and Zhejiang regions, especially in early-sowed single-season late rice seedlings. The disease is showing an outbreak trend in the rice producing areas of Jiangsu, Zhejiang and Henan. For example, in 2007, black-streaked dwarf disea...

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Problems solved by technology

For example, in 2007, black-streaked dwarf disease of rice first occurred in Jiangsu japonica rice, and the severely diseased fields had no harvest. In 2008, the affected area reached 2.67×10 5 hm 2 , and further increased to 3.33×10 in 2009 5 hm 2 , causing serious economic losses to rice production in Jiangsu

[0003] At present, the method of preventing and controlling rice black-streaked dwarf disease is mainly to use pesticides to prevent and control the vector SBPH. However, due to the large population of the vector, the control is difficult. Drug resistance leads to poor control effects, and the use of pesticides has the problem of environmental pollution. Therefore, mining resistant resources and cultivating disease-resistant varieties is the most economical and effective means to prevent and control the disease

Although some resistance QTLs have been found so far, due to the lack of disease resistance resources for rice black-streaked dwarf disease, the resistance presents quantitative genetic characteristics, and the resistance includes insect resistance and disease resistance, so far there is no resistance QTL. The report of sex gene being cloned

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