RNA (ribonucleic acid) extract liquid, preparation method and extraction method of oil palm fruit pericarp RNA

An extraction method and extraction solution technology, applied in the direction of DNA preparation, recombinant DNA technology, etc., can solve the problems of serious RNA degradation, difficult research and analysis, DNA pollution, etc., and achieve the effect of simple preparation method, good quality RNA, and less degradation

Pending Publication Date: 2018-07-27
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if the RNA can be extracted, it still contains a lot of impurities, making it difficult to carry out subsequent research and analysis
Although the CTAB method can be used to extract R

Method used

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  • RNA (ribonucleic acid) extract liquid, preparation method and extraction method of oil palm fruit pericarp RNA
  • RNA (ribonucleic acid) extract liquid, preparation method and extraction method of oil palm fruit pericarp RNA
  • RNA (ribonucleic acid) extract liquid, preparation method and extraction method of oil palm fruit pericarp RNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of RNA extraction liquid, the RNA extraction liquid of every 100ml comprises:

[0034]

Embodiment 2

[0036] A kind of RNA extraction liquid, the RNA extraction liquid of every 100ml comprises:

[0037]

[0038]

Embodiment 3

[0040] A kind of RNA extraction liquid, the RNA extraction liquid of every 100ml comprises:

[0041]

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PUM

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Abstract

The invention relates to the technical field of biology, and discloses RNA extract liquid, a preparation method and an extraction method of oil palm fruit pericarp RNA. The RNA extract liquid is prepared from common reagents, the preparation method is simple, and the quality is stable and controllable. The RNA of the oil palm fruit pericarp is extracted by adopting the RNA extract liquid disclosedby the invention, the obtained oil palm fruit pericarp RNA has good integrity, good quality, less degradation and less impurities, and the extract time is greatly shortened.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an RNA extraction solution, a preparation method and an oil palm fruit RNA extraction method. Background technique [0002] Oil palm (scientific name: Elaeis guineensis Jacq.) is a perennial monocot and is a tropical woody oil crop. The pulp and kernel of oil palm are rich in oil. Among various oil crops, it is known as the "world oil king". [0003] Because oil palm fruit contains a lot of pigments, oils, polysaccharides and other ingredients, it is difficult to extract RNA. RNA with high purity, good integrity, and no impurity contamination is the basis for molecular biology research such as cloning and cDNA library construction. Some commonly used RNA extraction kits, including Invitrogen Trizol and Tiagen's RNAplant, are not suitable for the extraction of RNA from oil palm fruit. During the extraction process, the RNA is severely degraded and the extraction efficiency is extre...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 肖勇夏薇周丽霞杨耀东李静
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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