Disposable Alternating Tangential Flow Filtration Unit
A single-use, tangential flow technology for filter separations, cartridge filters, pumps with flexible working elements, etc.
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example 2
[0086] Example 2 - Preparation of a single-use ATF device and operation of the ATF device to achieve membrane wetting
[0087] refer to Figure 6B , the following is a procedure designed to quickly and efficiently flush hollow fiber membranes using ATF technology. This flushing benefits the user by wetting, conditioning, reducing endotoxins and removing total organic compounds (TOC) from the membrane in a way. Using ATF has advantages over other methods because AT requires less volume and can be easily performed in a closed sterile system without using solutions incompatible with cell culture growth. ATF run rates are provided for various sizes of disposable ATF units.
[0088] program
[0089] 1. Connect the "T" tubing bank to the Feed 401 port, Retentate 402 port and Upper Permeate 403 port. The upper permeate port can be, for example, the upper port 102 shown in FIG. 1 .
[0090] 2. Connect a reservoir with wetting agent to the "T" outlet of port 401.
[0091] 3. ...
example 3
[0098] Example 3 - Filter Integrity Testing Using Regulated ATF Disposables
[0099] Also refer to Figure 6B , Filtration integrity testing is a means of ensuring that hollow fiber membranes are intact and that the membrane pores are fully wetted. After flushing in ATF mode, turn on the air source to 1 psi-2 psi by gravity or by reversing pump P2 to raise the diaphragm and open all other valves to drain the wetting fluid (media) back into the wetting fluid bag. The bag should be positioned to allow drainage.
[0100] After venting, close the air supply valve and relieve any pressure on the diaphragm. Then, close the valve located between the wetting agent bag and port 401 and the valve connected along the line to the retentate port 402, and remove the tubing from pump P1. Use an air source to slowly pressurize the feed side of the filter. Close the valve between the filter and the air pressure source and determine the pressure decay versus time. Correlate this with air...
example 4
[0102] Example 4 - Operating a SU-ATF with a C410 Controller for Diaphragm Life Testing
[0103] A disposable ATF6 with a 0.2 μm Spectrum PES filter was connected to a Repligen C410 controller. Connect the A2B connector to a 40°C water bath and run 500,000 pressure and 500,000 vent cycles at an ATF rate of 17.2 liters per minute (LPM). The permeate is pulled to 5.7LMH as it will be used in the perfusion process to keep operations as similar to bioreactor conditions as possible. This results in approximately 10,000 pressure and 10,000 exhaust cycles per day. The ATF was run for 50 days while monitoring diaphragm displacement. Displacement remained constant throughout the 50-day run, maintaining a constant flow as expected.
[0104] Example 5 - Production of monoclonal antibodies in a CHO perfusion bioreactor with SU-ATF2
[0105] Cells cultured using the ATF filter of the present disclosure were tested and the results are shown in Figure 8 and Figure 9 middle. During...
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