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Scopolamine taken as biomarker of toxic honey and application of scopolamine to indentify toxic honey

A biomarker, scopolamine technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unclear material basis, lack of systematic research on identification and identification methods, etc., and achieve high specificity and sensitivity Effect

Inactive Publication Date: 2018-08-10
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are related reports about Datura honey poisoning incidents at home and abroad, but there is a lack of systematic research on related identification and identification methods
[0004] At present, the material basis of human poisoning caused by the poisonous honey from Datura is still unclear

Method used

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  • Scopolamine taken as biomarker of toxic honey and application of scopolamine to indentify toxic honey
  • Scopolamine taken as biomarker of toxic honey and application of scopolamine to indentify toxic honey
  • Scopolamine taken as biomarker of toxic honey and application of scopolamine to indentify toxic honey

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Embodiment 1

[0036] Test case:

[0037] 1. Test materials: flowers, nectar and poisonous honey of yellow datura;

[0038] Among them, Datura nectar and poisonous honey are mainly obtained from the Datura planting base in Xiangyun County, Yunnan Province.

[0039] During the flowering period of Datura from May to July, pick the flowers of Datura, and use a micropipette to suck the nectar of Datura. In addition, in order to obtain Datura honey, 4 boxes of bees, 2 boxes of Italian bees, and 2 boxes of middle bees were placed 15 days before the flowering period of the Datura planting base, and the honey and pollen in the comb were removed before placement. , After the beehives are placed for 2 weeks, let the bees collect enough honey and store it in the comb, then collect 100-500g of honey for each beehive, and destroy the excess honey in the comb.

[0040] 2. Purchase 20 common honey samples from the market or beekeepers, such as rapeseed honey, acacia honey, jujube flower honey, vitex hone...

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Abstract

The invention relates to the field of food safety, and specifically discloses scopolamine taken as a biomarker of toxic honey and an application of scopolamine to indentify toxic honey. It is found through research that the content of a toxic atropine substance in datura stramonium in normal cognition is low in datura-stramonium-source honey, but scopolamine, the toxicity and content of which exceed that of atropine, becomes the key toxication component of datura-stramonium-source toxic honey. Therefore, scopolamine is especially suitable for recognition and identification of datura-stramonium-source toxic honey and confirmation diagnosis of the group poisoned by honey. On this basis, the invention further provides a method for detecting and analyzing UHPLC-Q Exactive plus in scopolamine of a honey sample, and the pre-treatment condition and detection parameters of the honey sample are optimized. The provided biomarker and detection method of toxic honey has great practical significance on protection of life health of honey consumers and maintenance of sound development of honey consuming industry.

Description

technical field [0001] The invention relates to the field of food safety, in particular to scopolamine as a biomarker of poisonous honey and its application in identifying poisonous honey. Background technique [0002] Honey is the nectar collected by bees from the flowers of flowering plants mixed with their own secretions, fully brewed and stored in the hive. Honey is a traditional natural sweet food. In addition to containing more sugars, it also contains a variety of flavonoids, phenolic acids, vitamins, amino acids and a variety of trace elements necessary for human beings. The health care function is deeply loved by consumers. However, in recent years, many honey poisoning incidents have broken out successively in many regions of my country. According to incomplete statistics, 13 honey poisoning incidents broke out in my country during the four years from 2014 to 2017, involving as many as 95 poisoned people. There were 15 people who died, and the fatality rate was as...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 杨术鹏张静张金振李熠周金慧金玥袁媛王鹏赵文
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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