A kind of quality detection method of Dendrobium fringe

A quality inspection method, the technology of Dendrobium fringe, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that cannot fully reflect the overall curative effect, achieve pertinence and applicability of interpretation, accurate quality evaluation, and solve the disaster of dimensionality Effect
CN108398516BActive Publication Date: 2020-12-15北京蓝标一成科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京蓝标一成科技有限公司
Publication Date
2020-12-15

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Abstract

The invention discloses a quality detection method of Dendrobium fimbriatum Hook. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the variety of a to-be-detected Dendrobium herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and / or naringenin as a reference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium fimbriatum Hook; and 4) establishing an analysis model by using the content value of the schaftoside and / or naringenin in the chromatographic data as the response variable and using the peak area value of other components in thefingerprint as the independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component. According to the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium fimbriatum Hook is precisely identified and controlled.
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Description

technical field

[0001] The invention belongs to the field of detection and analysis of traditional Chinese medicine components, and in particular relates to a quality detection method of dendrobium fringe. Background technique

[0002] Dendrobium fimbriatum (scientific name: Dendrobium fimbriatum Hook.): thick stem, erect or drooping, hard texture, cylindrical or sometimes slightly spindle-shaped above the base, 50cm-85cm long, 0.8cm-1.5cm in diameter, unbranched, with Multi-noded, light yellow or light yellow-brown after drying, internodes 3.5cm-4.8cm long, with many longitudinal grooves; leaf bases have leathery sheaths tightly hugging stems. Racemes 5cm-15cm long, 6-12 flowers sparsely; inflorescence rachis slender, somewhat curved; inflorescence peduncle 2cm-4cm long, base covered by several sheaths; sheath leathery, tubular, located at base The shortest, about 0.3cm long, the longest at the top, up to 1cm; bracts are membranous, ovate-triangular, 0.3cm-0.5cm long, with...

Claims

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