Primary cell extraction equipment and use method thereof

Abstract

The invention discloses primary cell extraction equipment and a use method thereof. The primary cell extraction equipment comprises a sample chopping zone, a working zone, an extraction zone and a sliding rail device penetrating through the three zones, wherein the sample chopping zone, the working zone and the extraction zone are sequentially adjacent, the sample chopping zone comprises a tissuechopping device, the working zone comprises a first gate, a heating temperature control device, a rotary clamping device, a centrifugation device, a cover opening / closing device, a test tube placementframe and a pipetting device, the first gate is adjacent to the sample chopping zone, the heating temperature control device is adjacent to the first gate and is sequentially adjacent to the rotary clamping device and the centrifugation device, the cover opening / closing device is adjacent to the first gate, is opposite to the heating temperature control device, and is sequentially adjacent to thetest tube placement frame and the pipetting device, the extraction zone contains a second gate adjacent to the working zone, and the sliding rail device has a trolley capable of sliding on the sliding rail device. With the primary cell extraction equipment of the present invention, the manpower can be saved, and the pollution can be reduced.

Description

technical field [0001] The invention relates to an extraction device, especially a cell extraction device. The present invention also relates to a method of use, in particular to a method for implementing the aforementioned primary cell extraction device. Background technique [0002] In recent years, the technology of extracting primary cell culture has developed quite maturely, and the continuous cultivation of primary cells has been applied in various fields, so that the extraction of primary cells is currently urgently needed to be mass-produced. [0003] In the prior art, since the extraction of primary cells involves multiple steps, it is necessary to hire multiple technicians to start with obtaining the tissue, and perform multiple procedures such as chopping, centrifugation, enzyme reaction, washing, and culturing in a laboratory. Steps, not only the size of the tissue cut by each technician is different, but also need to move back and forth between the steps repeat...

AI Technical Summary

Problems solved by technology

It often takes more than half a working day from obtaining the tissue to starting the culture. Once a large amount of primary cells needs to be extracted, it is necessary to hire multiple technicians at the same time.

[0004] However, employing multiple technicians will not only increase the cost, but also because there are many procedures in the extraction steps of the primary cells, it may not be operated in a sterile room or a laminar flow cabinet. Entering and exiting the sterile room or laminar flow cabinet will not only greatly increase the possibility of contamination of the primary cells, but also lack efficiency

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