Method for detecting secretion functions of cells
A cell secretion and cell secretion technology, which is applied in the field of detection of cell secretion function, can solve the problems of lower detection accuracy and cell changes, and achieve objective results, eliminate interference, and improve accuracy.
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Embodiment 1
[0025] The present invention proposes a method for detecting cell secretion function, comprising the following steps:
[0026] S1: Select the cells to be tested, place the cells to be tested in a sterile storage room, and add the first cell culture medium to the sterile storage room, and discard the first cell culture medium when the cells to be tested are in the logarithmic division phase ;
[0027] S2: Take out the cells to be tested, wash the cells to be tested with buffer solution for three times, then add the second cell culture medium, and culture the cells again, and the culture time is 48 hours, respectively take 350 μL and 600 μL of cell culture medium with different culture time Mix absolute ethanol in a centrifuge tube, then centrifuge for 6 minutes, discard the supernatant to obtain the cell secreted protein precipitated at the bottom of the centrifuge tube, then dry the cell secreted protein, and use acetone after drying Cell secreted protein was fixed for 5 minu...
Embodiment 2
[0044] The present invention proposes a method for detecting cell secretion function, comprising the following steps:
[0045] S1: Select the cells to be tested, place the cells to be tested in a sterile storage room, and add the first cell culture medium to the sterile storage room, and discard the first cell culture medium when the cells to be tested are in the logarithmic division phase ;
[0046] S2: Take out the cells to be tested, wash the cells to be tested with the buffer solution three times, then add the second cell culture medium, and culture the cells again, and the culture time is 50 h, respectively take 380 μL and 650 μL of the cell culture medium with different culture time Mix absolute ethanol in a centrifuge tube, then centrifuge for 7 minutes, discard the supernatant, and obtain the cell secreted protein precipitated at the bottom of the centrifuge tube, then dry the cell secreted protein, and use acetone after drying Cell secreted protein was fixed for 6 mi...
Embodiment 3
[0063] The present invention proposes a method for detecting cell secretion function, comprising the following steps:
[0064] S1: Select the cells to be tested, place the cells to be tested in a sterile storage room, and add the first cell culture medium to the sterile storage room, and discard the first cell culture medium when the cells to be tested are in the logarithmic division phase ;
[0065] S2: Take out the cells to be tested, wash the cells to be tested with buffer solution three times, then add the second cell culture medium, and culture the cells again, and the culture time is 52 hours, respectively take 420 μL and 750 μL of cell culture fluids for different culture times Mix absolute ethanol in a centrifuge tube, then centrifuge for 8 minutes, discard the supernatant to obtain the cell secreted protein precipitated at the bottom of the centrifuge tube, then dry the cell secreted protein, and use acetone after drying Cell secreted protein was fixed for 7 minutes ...
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