Method of splitting and determining metolachlor enantiomer as chiral pesticide with ultra performance bonded phase chromatography-tandem mass spectrometry technology

Abstract

The invention belongs to the analytical chemistry field and the technical field of pesticide residue detection and particularly discloses a method of splitting and determining a metolachlor enantiomeras a chiral pesticide with an ultra performance bonded phase chromatography-tandem mass spectrometry technology. In the method, a QuEChERS method is adopted to extract metolachlor in tobaccos, grainsand dried fruits, an enantiomer of the metolachlor chiral pesticide is detected by combining bonded phase chromatography chiral stationary phase with triple stage quadrupole tandem mass spectrometry,and a method detected limit is 0.0012mg / kg. The method adopts bonded phase chromatography to rapidly perform chiral separation on the metolachlor for the first time; and by taking supercritical CO2 as a mobile phase, use of a great amount of an organic solvent is saved, and the method is green and environmentally friendly. The method uses bonded phase chromatography for rapid analysis, is beneficial to rapid separation and analysis speed of an isomer, consumes 4mins only and is high in sensitivity.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and the technical field of pesticide residue detection, and relates to a method for the resolution and quantification of enantiomers of chiral pesticides. Method for enantiomers of alachlor. Background technique [0002] 25% of the currently used pesticides are chiral, and there are enantiomeric differences in the biological activity of chiral pesticides, and their activity often only exists in one or a few enantiomers, while the enantiomeric Dissolution and fate in the environment are often also significantly different. That is, there may be huge differences in the activity, toxicity, absorption, metabolism, and degradation of chiral pesticide enantiomers in the natural environment and organisms. [0003] Metolachlor is an amide weed control agent, which can be widely used as a weed control agent in more than 70 crop fields because of its broad-spectrum, high efficiency, and strong selectivi...

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Problems solved by technology

[0005] At present, there are reports on the separation of metolachlor isomers using liquid chromatography chiral stationary phase [Analytical Chemistry Research Bulletin, 2004, 32(2), 191-194; Pesticides, 2008, 47(9), 655- 656], these are all separated by high performance liquid chromatography-chiral chromatographic column, the separation time is long, and there is no quantitative analysis method established for the implementation of product quality control

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