A method for detecting a target using an antibody modified with a chemiluminescence enhancer

Abstract

The present invention discloses a method of modifying an enhancer of a chemiluminescent substrate onto an antibody to detect the concentration and the like of a target. The method comprises the following steps: antibody 1 is labeled with a compound having an enhancing effect on a chemiluminescence signal, and antibody 2 is labeled with a chemiluminescent catalytic substance such as horseradish peroxidase, alkaline phosphatase or the like; luminal, a peroxide and other luminescent substrates are dissolved in a solution, when the target to be tested is in the solution, the antibody 1 and the antibody 2 are combined with the target to be tested, the catalytic substance and the enhancer are close in space to enhance the chemiluminescence effect. The method is a test method which is homogeneous, and does not require separation and purification and other steps.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to a method for detecting a target by using an antibody modified by a chemiluminescent enhancer. Background technique [0002] Immunoassay is an important detection method in the field of in vitro diagnostic reagents. The main detection object is a variety of proteins (antigens), mainly using two kinds of antibodies and antigens to form a "sandwich" structure to achieve the purpose of detection. At present, the vast majority of immunological methods use solid phase carriers (such as 96-well plates, mold strips, magnetic beads, chips, etc.) to immobilize one antibody, while the other antibody is generally labeled with a tracer substance (such as enzyme, fluorescent molecules, colored molecules, etc.). After forming a sandwich structure with the tested antigen, the antigen and antibody not bound to the solid-phase carrier are washed away. Quantitative determination of the remai...

AI Technical Summary

Problems solved by technology

There are certain defects in this method: the first is that the existence of the solid phase carrier mentioned above increases the complexity of the instrument, and the second is that the washing process is still required, which makes the detection process very complicated

Although this method does not require washing steps, it also has obvious defects. The first is that the amount of luminescent substrate is very small, and the luminescence ends soon, which is very unfavorable for the signal reception of the instrument. The total number of emitted photons is small, so The sensitivity is low; the second is that unlike the classic and mature enzyme labeling technology, it is more technically difficult to effectively label the luminescent substrate to the antibody, which makes this method more difficult and costly

Moreover, the luminescent substrate suitable for this method is very special, and the synthesis is very difficult, which is not conducive to the promotion and application of this method.

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