Method for representing plasma induced intracellular absolute calcium ion concentration
A plasma-induced cell technology, applied in the field of quantitative detection of intracellular calcium ion concentration, to achieve wide application prospects, accurate measurement and characterization
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[0037] A method for characterizing the absolute intracellular calcium ion concentration induced by plasma comprising the steps of:
[0038] Step 1, Prepare Cell Suspension
[0039] Saccharomyces cerevisiae CGMCC no.6148 was cultured in YPD culture medium (10g / L yeast extract powder, 20g / L peptone, 20g / L glucose) at 28°C, 170rpm; collect Saccharomyces cerevisiae cells in logarithmic growth phase, centrifuge at 4°C, 30000 ×g, 5min, discard the supernatant, resuspend in fresh 2% YPD culture medium, adjust the bacterial concentration OD 600 to 6.0 (at this point cell concentration = 5 × 10 5 individual / mL).
[0040] Step 2, optimize the use conditions of fluo-3AM
[0041] (1) Optimization of water bath temperature and incubation time
[0042] Divide the cell suspension prepared in step 1 into 1.5mL centrifuge tubes, 1mL in each tube, 6 tubes in total, and each 6 tubes is a group, and each group is treated in a water bath at 30°C, 37°C, and 44°C respectively 0min, 20min, 40min...
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