A Method for Characterizing Absolute Intracellular Calcium Ion Concentrations Induced by Plasma
A technology of plasma and induced cells, applied in the preparation of test samples, material excitation analysis, fluorescence/phosphorescence, etc., to achieve wide application prospects, accurate measurement and characterization
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[0038] A method for characterizing the absolute intracellular calcium ion concentration induced by plasma comprising the steps of:
[0039] Step 1, Prepare Cell Suspension
[0040] Saccharomyces cerevisiae CGMCC no.6148 was cultured in YPD culture medium (10g / L yeast extract powder, 20g / L peptone, 20g / L glucose) at 28°C, 170rpm; collect Saccharomyces cerevisiae cells in logarithmic growth phase, centrifuge at 4°C, 30000 ×g, 5min, discard the supernatant, resuspend in fresh 2% YPD culture medium, adjust the bacterial concentration OD 600 to 6.0, when the cell concentration is 5×10 5 individual / mL.
[0041] Step 2, Optimizing Fluo-3 AM Use Conditions
[0042] (1) Optimization of water bath temperature and incubation time
[0043] Divide the cell suspension prepared in step 1 into 1.5mL centrifuge tubes, 1mL in each tube, 6 tubes in total, and each 6 tubes is a group, and each group is treated in a water bath at 30°C, 37°C, and 44°C for 0 min , 20min, 40min, 60min, 80min, 10...
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