Preparation method and application of interlayer type lung cancer marker electrochemical transducer

Abstract

The invention belongs to the technical field of immunoassay and biological sensing technologies and provides a preparation method and application of an interlayer type lung cancer marker electrochemical transducer. Graphene-loaded Cu2+ doped by functional nitrogen of dual-metal nanometer material Au@Pt of a core-shell structure is utilized as a catalytic material and serves as a marker after beinghatched with detected antibodies, and meanwhile nanometer gold particles functionalized by polydopamine are adopted as electrode modification materials, so that detection of lung cancer marks such asCEA, CA125 and SCCA is achieved. The interlayer type lung cancer marker electrochemical transducer has high sensitivity and specificity, low detection limitations and a significant scientific meaningand a high application value on early detection of lung cancer.

Description

technical field

[0001] The invention belongs to the technical field of immune analysis and biosensing, and relates to a preparation method and application of a sandwich-type electrochemical sensor for lung cancer markers. Specifically, new nano-functional materials are used as catalysts to label detection antibodies to achieve sensitive detection of lung cancer markers CEA, CA125, and SCCA. Background technique

[0002] Lung cancer has a high incidence rate, is difficult to detect, and the tumor grows and metastasizes rapidly, becoming one of the malignant tumors that threaten the health and life of the population most. Therefore, it is very important to realize the sensitive detection of lung cancer markers for the early detection and early treatment of lung cancer. At present, there are many detection methods for lung cancer markers, such as enzyme-linked immunoassay, radioimmunoassay, etc., but these methods may require large-scale instruments and equipment during the de...