Plant cultivation method using metal nanoparticles and nutrient medium applied thereto

A technology of nano-particles and cultivation methods, applied in the field of biology and nanometers in plant cultivation, capable of solving problems such as destroying cells

Active Publication Date: 2020-10-20
SHENZHOUSPACEBIOTECHGRP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, carbon nanotubes are known to penetrate the human body through the skin, through inhalation or oral administration, and damage cells like asbestos

Method used

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  • Plant cultivation method using metal nanoparticles and nutrient medium applied thereto
  • Plant cultivation method using metal nanoparticles and nutrient medium applied thereto
  • Plant cultivation method using metal nanoparticles and nutrient medium applied thereto

Examples

Experimental program
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preparation example Construction

[0044] Regarding the preparation of the nutrient medium according to the present invention, any known balanced nutrient medium (containing all organic and inorganic macronutrients and micronutrients essential to plants) preparations can be used as a basis. Therefore, in order to prepare the nutrient medium according to the present invention, electrically neutral iron nanoparticles, electrically neutral zinc nanoparticles, or electrically neutral copper nanoparticles, or a combination thereof are used instead of iron salt, zinc salt or copper salt or these The combination of salts is introduced into the medium while the other components of the nutrient medium used as the basis remain unchanged.

[0045] In order to prove the possibility of implementing the present invention, the agar Murashing-Skoog nutrient medium preparation (see Table 1) is used as a basis to prepare the nutrient medium composition according to the embodiment of the present invention.

[0046] In the embodiment o...

Embodiment 1

[0099] Example 1: The effect of metal nanoparticles and chitosan in the nutrient medium composition on the germination of tomato seeds.

[0100] Seed germination is characterized by the value of germination index, which is defined as the percentage of the number of germinated seeds to the total number of seeds taken. The seed germination index was determined on the 15th day after the start of plant cultivation.

[0101] Figure 2A-2D It shows the effect of replacing metal salts with metal nanoparticles in the nutrient medium composition and supplementation of chitosan (100 mg / L) on the germination of Venice tomato seeds.

[0102] Figure 2A It was shown that on a nutrient medium containing iron nanoparticles with a concentration of 0.6-10.0 mg / L, the germination index of tomato seeds increased by 13-27% (compared to the control group). The addition of chitosan to the nutrient medium containing iron nanoparticles showed an additional increase of 23-33% in germination index.

[0103] ...

Embodiment 2

[0106] Example 2: The effect of metal nanoparticles in the nutrient medium composition on the morphological indicators of pepper and tomato plants ring.

[0107] The morphological indicators of plants (germination length, root length, and green part biomass yield) were measured on the 40th day after the plant was cultured on a nutrient medium containing metal nanoparticles (under the above conditions). The results are expressed as a percentage between the index obtained in the experiment (test group) and the control group.

[0108] Figure 3A-3D The effect of metal nanoparticles in the nutrient medium composition on the root length of LJ-king pepper is shown.

[0109] From Figure 3A It can be seen that, compared with the control group, the nutrient containing iron nanoparticles with concentrations of 0.06mg / L, 0.3mg / L and 3.0mg / L instead of ionic iron (5.6mg / L as metal) Cultivating pepper on the medium resulted in a 54%, 118% and 102% increase in root length. This positive effec...

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PUM

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Abstract

The invention relates to the field of biology and nanotechnology in plant cultivation, which can be used to produce high-quality crop planting materials for the implementation of biotechnology research, thereby improving the quality of agricultural products. The invention can be used in aerosol culture and hydroponics, and can also be used to create a life support system for astronauts under long-term space flight conditions. The proposed plant culture method involves germinating seeds and subsequently cultivating plants on a sterile agar nutrient medium containing a set of organic and inorganic components essential for plant growth, including: iron, zinc and copper, where iron, Zinc or copper or combinations thereof are in the form of nanoparticles of electrically neutral metals. Additionally, the nutrient medium may include chitosan. The present invention is capable of improving seed germination and increasing physiological and morphological indicators of plants, such as root length and root activity, chlorophyll content of leaves, germination length and green part biomass yield.

Description

Technical field [0001] The invention relates to the fields of biology and nanotechnology in plant cultivation, and can be used to produce high-quality crop planting materials to guide biotechnology research, thereby improving the quality of agricultural products. The invention can be used in aerosol cultivation and hydroponic cultivation, and can also be used to create a life support system for astronauts under long-term space flight conditions. Background technique [0002] The application of nanomaterials as plant protection agents and fertilizers can help improve the resistance of plants to adverse weather conditions and diseases, and increase the yield and quality of crops. Nanotechnology preparations (for example, NanoGro, GreenLift, AgBion, etc.) are known, but research on the field of development of new preparations and methods to improve the production efficiency of agricultural products using nanotechnology is still continuing. In the review article, the latest progress...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/00A01G31/00A01G24/10A01G24/30
CPCA01G22/00A01G24/10A01G31/00A01N59/16A01N59/20B82Y5/00A01G22/05
Inventor 赵辉刘敏陈瑜鹿金颖李华盛孙乔G·S·尼奇塔洛A·N·日加奇I·O·利普尼斯基A·A·雅克米托娃O·A·博格斯洛夫斯卡N·N·格卢先科
Owner SHENZHOUSPACEBIOTECHGRP
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